白藜芦醇对离体豚鼠乳头肌电生理效应.pdf

708 Acta Physiologica Sinica , December 25, 2004, 56 (6): 708-712 Research Paper Electrophysiological effects of resveratrol on guinea pig papillary muscles ZHAO Juan, MA Hui-Jie, DONG Jing-Hui, ZHANG Li-Ping, LIU Hua-Lei, WANG Qing-Shan* Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazh uang 050017, China Abstract: The purpose of this study was to investigate the electrophysiological effects of resveratrol on guinea pig papillary muscles and the underlying mechanism. Action potentials were recorded by using intracellular microelectrode technique. The results obtained are as follows: (1) In normal papillary muscles, resveratrol (30, 60, and 120 µmol/L) shortened the duration of action potential (APD) in a concentration-dependent manner. (2) In partially depolarized papillary muscles, resveratrol ( 60 µmol/L ) not only shortened APD, but also decreased the amplitude of action potential ( APA ), overshoot ( OS ) and maximal rate of depolarization in phase 0 ( V ). (3) max Perfusion with Ca2+-free K-H solution, completely abolished the effects of resveratrol (60 µmol/L) on papillary muscles. (4) Applica- tion of potassium channel blocker tetraethylammonium chloride (TEA, 20 mmol/L) did not prevent the effect of resveratrol (60 µmol/L) on action potential. (5) Pretreatment with NG-nitro-L -arginine methyl ester (L -NAME, 1 mmol/L), a nitric oxide (NO) synthase inhibitor, failed to abolish the effect of resveratrol (60 µmol/L). All these results indicate that the electrophysiological effects of resveratrol on guinea pig papillary muscles are likely due to the reduction of calcium influx, which might not be mediated by NO. Key wo