非放射性杂交方法 Non-Radioactive ISH 英文.pdfVIP

11/20/2002 Non radioactive in situ hybridization The following is a non-radioactive in situ protocol for plants, using an RNA probe. It is derived from several protocols, compiled and worked out by Cindy Lincoln and then slightly modified by myself. The fixation/dehydration/embedding portion is derived from a protocol from Elliot Meyerowitzs lab. The RNA probe synthesis is from David Jackson. The actual in situ section is from both David Jackson and V