基于分子生物学与基因组学的植物根际微生物研究.PDF

微生物学通报 JUN 20, 2009, 36(6): 892~899 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 专论与综述 基于分子生物学和基因组学的植物根际 微生物研究 1* 2 2 郝大程 陈士林 肖培根 (1. 116028) (2. 100193) 摘 要: 根际微生物是指在植物根系直接影响的土壤范围内生长繁殖的微生物。根际微生物研究 对微生物生态学和工业生物技术开发均十分重要, 妨碍其研究进展的主要原因是 99%的根际微生 物物种在实验室中无法成功培养。近年来, 将基于分子生物学和基因组学的非培养研究技术应用 于根际微生物多样性研究取得了很大进展, 本文结合作者对红豆杉根际的研究, 评述近期相关新 技术的应用和其未来应用前景。 关键词: 根际微生物, 分子生物学, 基因组学, 系统发育, 多样性 Study of Rhizosphere Microbe Based on Molecular Biology and Genomics HAO Da-Cheng1* CHEN Shi-Lin2 XIAO Pei-Gen2 (1. Laboratory of Biotechnology , Dalian Jiaotong University , Dalian , Liaoning 116028, China) (2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China) Abstract: The rhizosphere is the narrow region of soil that is directly influenced by roots and associated soil microorganisms. Research on rhizosphere microbe is essential for microbial ecology and industrial biotech- nology. However, the inability of culturing most rhizosphere microorganisms (around 99%) in the laboratory obviates the research progress. In recent years, there is enormous advance in applying non-culturing tech- niques based on molecular biology and genomics to the study of rhizosphere microbial diversity and plant-microbe interactions. This review discusses recent findings and future challenges in the study of rhizosphere microbe, with brief comment of our Taxus rhizo