广州市某医院周边鼠形动物肺炎克雷伯菌与铜绿假单胞菌的耐.PDF

·688 · doi 10.3969/j.issn. 1673-4254.2016.05.17 J South Med Univ, 2016, 36(5): 688-692 流行病学调查 广州市广州市某医院周边鼠形动物肺炎克雷伯菌和铜绿假单胞菌的耐某医院周边鼠形动物肺炎克雷伯菌和铜绿假单胞菌的耐 药药性调查性调查 钟雪珊，葛 婧，陈少威，熊益权，郑雪燕，邱 旻，霍舒婷，陈 清 南方医科大学公共卫生与热带医学学院流行病学系，广东 广州 510515 摘要：目的 了解鼠形动物粪便标本中携带肺炎克雷伯菌与铜绿假单胞菌的耐药情况。方法 2015 年3 月~ 10月，利用笼捕法于 广州市某医院及其周边居民区捕获鼠形动物，分离其粪便中肺炎克雷伯菌与铜绿假单胞菌菌株，按照2014 年CLSI 标准进行药 敏试验，利用SPSS 20.0 软件分析数据。结果 共捕获鼠形动物329 只（包括205 只臭鼩鼱、111只褐家鼠、5 只黄胸鼠和8 只小家 鼠）。肺炎克雷伯菌与铜绿假单胞菌在鼠形动物体内检出率分别为78.4%和34.7%。臭鼩鼱携带的肺炎克雷伯菌对氨苄西林、 头孢唑林、呋喃妥因、哌拉西林和头孢噻肟耐药性高（耐药率分别为100%、51.2%、44.2%、37.2%和23.3%）；家鼠携带肺炎克雷伯 菌耐药情况与鼩鼱相似。肺炎克雷伯菌的多重耐药率为40.9% ，产ESBLs 菌株检出率为10.7%。鼩鼱与家鼠携带的铜绿假单胞 菌均对氨曲南耐药情况最严重（耐药率为12.4%和16.0%），其次为广谱青霉素类和喹诺酮类抗生素，对头孢菌素、氨基糖苷类和 碳青霉烯类抗生素耐药率最低（耐药率均5%）。结论 鼠形动物携带的肺炎克雷伯菌与铜绿假单胞菌的耐药谱与目前临床分 离株的耐药谱相似，提示这两种细菌可能在鼠形动物与人之间有传播作用，值得进一步研究。 关键词：家鼠；鼩鼱；铜绿假单胞菌；肺炎克雷伯菌；抗生素耐药性 Investigation of antimicrobial resistance of Klebsiella pneumoniae and Pseudomonas aeruginosa isolates from rat-like animals around a hospital in Guangzhou ZHONG Xueshan, GE Jing, CHEN Shaowei, XIONG Yiquan, ZHENG Xueyan, QIU Min, HUO Shuting, CHEN Qing Department of Epidemiology, School of Public Health and Tropical Medicine, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou 510515, China Abstract: Objective To investigate antimicrobial resistance of Klebsiella pneumoniae and Pseudomonas aeruginosa isolates in fecal samples from rat-like animals. Methods Rat-like animals were captured using cages around a hospital and the neighboring residential area between March and October, 2015. K. pneumoniae and P. aeruginosa were isolated from the fecal samples of the captured animals. Antimicrobial susceptibility test was performed according to the guidelines of Clinical and Laboratory Standards Institute (2014). Results A total of 329 rat-