pH值对沼液培养的普通小球藻生长和油含量积累的影响.PDF

生 物 工 程 学 报 Chin J Biotech 2010, August 25; 26(8): 1074−1079 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ ©2010 CJB, All rights reserved. 环境生物技术 pH 值对沼液培养的普通小球藻生长及油含量积累的 影响 王翠，李环，王钦琪，韦萍 南京工业大学 生物与制药工程学院，南京 210009 摘 要: 以 50 ％的沼液为普通小球藻的全营养培养基，考察培养基的起始pH 值对小球藻生长及油脂含量的影响，普 通小球藻对不同初始pH 的沼液中氮、磷的去除情况。设定了2 组实验，一组只调节初始接种培养液的pH ，分别为6.0 、 6.5 、7.0 、7.5 、8.0 、8.5；另一组将培养液pH 分别固定在6.0 、6.5 、7.0 、7.5 、8.0、8.5 ，pH 用稀HCl 和NaOH 进行调 节。研究发现在pH 6.5 和pH 7.0 的偏酸环境有利于小球藻生长，而pH 在7.0~8.5 的偏碱性条件下有利于小球藻油脂的 积累，因此综合小球藻生长和油脂积累2 个因素，得到最适合小球藻生长和油脂积累的pH 为7.0 。培养结束后沼液中 氮磷的去除率分别达到了95 ％和97 ％，沼液中的总氮由原来的134.91 mg/L 降至4.86 mg/L ，总磷由10.19 mg/L 降到 0.32 mg/L 。 关键词: 普通小球藻，pH ，沼液，油脂 Effect of pH on growth and lipid content of Chlorella vulgaris cultured in biogas slurry Cui Wang, Huan Li, Qinqi Wang, and Ping Wei College of Biotechnology and Pharmaceutical Engneering, Nanjing University of Technology , Nanjing 210009, China Abstract: Using 50% biogas slurry as basic medium, we investigated the effect of pH on the growth and lipid accumulation of Chlorella vulgaris. Setting two-group experiments, one was only control the initial medium pH, the initial pH was set at 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5, respectively. One was control the medium pH constant, set constant pH at 6.0, 6.5, 7.0, 7.5, 8.0, and 8.5, respectively. Using HCl and NaOH regulated the pH. Results showed that algae Chlorella vulgaris grows better at pH 6.5 and 7.0, accumulate the lipid at pH 7.0−8.5, so the optimal pH for the growth and the lipid accumulation of Chlorella vulgaris was 7.0. The average removal rate of nitrate from biogas slurry was 95%, phosphate was 97%. Keywords: Chlorella vulgaris, pH, biogas slurry, lipid 随着经济的不断发展，人类对能源的需求量越 染严重，以储量丰富且环境友好的生物质能源代替 来越