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Talanta 121 (2014) 210 –214 Contents lists available at ScienceDirect Talanta journal homepage: /locate/talanta Rapid monitoring of glycerol in fermentation growth media: Facilitating crude glycerol bioprocess development Sergi Abad, Xavier Pérez, Antoni Planas, Xavier Turon n Bioengineering Department, Institut Químic de Sarrià (IQS), Universitat Ramon Llull. Via Augusta 390, 08017 Barcelona, Spain a r t i c l e i n f o a b s t r a c t Article history: Recently, the need for crude glycerol valorisation from the biodiesel industry has generated many studies Received 16 July 2013 for practical and economic applications. Amongst them, fermentations based on glycerol media for the Received in revised form production of high value metabolites are prominent applications. This has generated a need to develop 5 December 2013 analytical techniques which allow fast and simple glycerol monitoring during fermentation. The Accepted 11 December 2013 methodology should be fast and inexpensive to be adopted in research, as well as in industrial appli- Available online 9 January 2014 cations. In this study three different methods were analysed and compared: two common methodologies Keywords: based on liquid chromatography and enzymatic kits, and the new method based on a DotBlot assay Monitoring