微粒说明书MEGAMIX-PLUS#FSC_.pdfVIP

Megamix-Plus FSC Beads for cytometer settings in microparticle analysis Kit for 50 Tests Ref. 7802 For Research Use Only. Not for Use in Diagnostic Procedures. 1 INTRODUCTION 5 REAGENT PREPARATION AND STORAGE Biological microparticles (MP) are cellular vesicles of heterogeneous size Unopened vial remains stable until expiration date printed on the label when ranging from 0.1 to 1 µm. Due to such small size, MP analysis requires stored at 2-8°C. Do not freeze. working conditions close to the size-related sensitivity limit of the The reagent is ready for use. cytometers Standardization of MP count requires to master this limit for an optimal compromise between MP analysis and background exclusion This Resuspend this reagent by vortexing for 10 seconds before use. limit has significantly progressed with the advent of the latest generations Stability after opening: until expiration date printed on the label at 2-8°C of instruments from 0.5 down to 0.3 µm-eq.* (ref.1). Also, experience with when free of contamination. Megamix have shown that FCM instruments may not all behave the same