s气相色谱法测定婴幼儿配方乳粉中1,3-二油酸2-棕榈酸甘油三酯.pdfVIP

JOURNAL OF INSPECTION AND QUARANTINE 检验检疫学刊 Vol.23 No. 6 2013 年第 6 期 气相色谱法测定婴幼儿配方乳粉中 1，3-二油酸2-棕榈酸甘油三酯 * 马小宁 田 燕 宋小莉 张凤霞 贾 迪 李淑娟 仲维科 刘汉霞 （中国检验检疫科学研究院 北京 100123 ） 摘摘要要 采用乙醚和石油醚提取出配方乳粉中的油脂，以C51:0为内标，通过带有多模式进样口进样器及氢 火焰检测器的气相色谱仪对其中的1,3-二油酸2-棕榈酸甘油三酯进行定量分析。结果表明，在本方法的色谱条 件下，配方乳粉的1,3-二油酸2-棕榈酸甘油三酯能与其他C52 系列脂肪酸组分很好的分离，方法重现性良好， RSD为2.93 % ，回收率为92.4 ±4.5% 。该方法准确可靠，可准确测定配方乳粉中OPO 的含量。 关关键词键词 婴幼儿配方乳粉；1,3-二油酸2-棕榈酸甘油三酯；气相色谱 （氢火焰检测器）；多模式进样口； 定量检测 中中图分类号图分类号 O657.71 Determination of 1, 3-Dioleoyl 2-Palmitoyl Triglyceride (OPO) in Infant Formula with Gas Chromatography Methods Ma Xiaoning, Tian Yan, Song Xiaoli, Zhang Fengxia, Jia Di, Li Shujuan, Zhong Weike, Liu Hanxia (Chinese Academy of Inspection and Quarantine, Beijing, 100123) Abstract: Establish quantification determination method for 1,3-Dioleoyl 2-palmitoyl triglyceride (OPO) in infant formula in order to meet the requirement of the product and to supply technical support for guarantee the infant formula safety. OPO in infant formula were extracted by ethyl ether and petroleum ether, and then OPO was determined by gas chromatography (GC) equipped with multimodal injector (MMI) sample injector and FID detector with C51:0 as internal standard. The results showed that OPO was separated from other C52 compounds very well under the GC condition. The reproducibility of the method is good, the relative standard deviation was 2.93 % and the recovery was 92.4±4.5%. The method was accurate and stable to determine the concentration of OPO in the infant formula. Key Words: Infant Formula; 1,3-Dioleoyl 2-Palmitoyl Triglyceride; Gas Chromatography(FID); Multimodal Injector; Quantifi cation Determination 1 前言 婴幼儿配方