农研铁皮石斛种植之石斛不同种部位中多糖含量测定.doc

农研铁皮石斛种植之石斛不同种部位中多糖含量测定目的测定不同种石斛及金钗石斛、马鞭石斛、叠鞘石斛、鼓槌石斛的不同药用部位中多糖的含量。方法采用紫外-可见分光光度法。结果不同种石斛茎中多糖含量为：球花石斛18.96 %，马鞭石斛24.89 %，金钗石斛20.49 %，报春石斛15.83 %，肿节石斛8.27 %，叠鞘石斛16.32 %，鼓槌石斛15.33 %，美花石斛28.28 %，兜唇石斛9.26 %；4种石斛不同药用部位多糖的含量：金钗石斛根15.36 %，茎20.49 %，叶22.30 %；马鞭石斛根17.56%，茎24.89 %，叶20.88 %；叠鞘石斛根9.48 %，茎16.32 %，叶17.42 %；鼓槌石斛根10.06 %，茎15.33 %，叶19.81 %。结论该实验方法简便、快速、准确。各种石斛及其根茎叶中多糖含量均比较高，从资源的综合和可持续利用的角度考虑，建议可以全草入药；美花石斛、球花石斛、报春石斛、叠鞘石斛、鼓槌石斛、肿节石斛茎在进行药效研究的提前下，确认是否可以替代当前《中国药典》品种入药。 　　Abstract：ObjectiveTo determine polysaccharide from several Herba Dendrobii and different medical parts of D. nobile Lindl, D. fimbriatum Hook, D. denneanum Kerr, and D. chrysotoxum Lindl.MethodsUV method was used.ResultsThe content of polysaccharides in different Herba Dendrobii was as follows: D. thyrsiflorum. Rchb.f of 18.96%, D.fimbriatum Hook of 24.89%, D. nobile Lindl of 20.49% , D. crepidatum Lindl of 15.83%, D. pendulum Roxb of 8.27%, D. denneanum Kerr of 16.32%, D. chrysotoxum Lindl of 15.33 %, D.loddigesii Rolfe of 28.28%, D．aphyllum (Roxb.)C. E. Fisch of 9.26%; the contents of polysaccharides from root, stem and leaf in D. nobile Lindl Lindl were 15.36% , 20.49% and 22.30%; In D.fimbriatum Hook were 17.56% , 24.89% , 20.88%; In D. denneanum Kerr were 9.48%, 16.32%, 17.42%; In D. chrysotoxum Lindl were 10.06%, 15.33%, 9.81%.ConclusionThis method is simple, sensitive and accurate. The content of polysaccharides is higher in leaves from different parts, and we should try to keep leaves when medicinal material is processed. 石斛属Dendrobium为兰科最大的属之一, 全世界共有一千五百多种, 广泛分布于亚洲、欧洲及大洋洲等热带及亚热带地区。我国约有76种,主要分布于西南、华东及华南地区。石斛首先记载于《神农本草经》，并列为上品。《本草纲目》言：“俗方最以补虚，主治伤中，除痹下气，补五脏虚劳羸瘦，强阴益精；厚肠胃，补内绝不足，逐皮肤邪热痒气，治男子腰脚软弱，健阳，补肾益力，壮筋骨，暖水脏，轻身延年等”在我国传统医学中经历了2000年以上的应用历史。《中国药典》2005年版收载金钗石斛Dendrobium nobile Lindl、铁皮石斛Dendrobium candidum Wall.ex. Lindl、马鞭石斛Dendrobium fimbriatum Hook。石斛具有滋阴清热、益胃生津和润肺止咳等功效, 常用于治疗热病伤津、口干烦渴、病后虚热等多种病症。现代药理研究证明石斛中多糖类成分是石斛具有免疫增强作用和抗肿瘤作用的活性成分 。 石斛a href= target=_blank()/a为我国常用贵重药材,野生资源匮乏，而种植石斛规模较小，不能满足