TNF-α与1型糖尿病.pdf

Eur. Cytokine Netw., Vol. 21 n° 4, December 2010, 285-91 285 RESEARCH ARTICLE Identification of specific tumor necrosis factor-α-susceptible and -protective haplotypes associated with the risk of type 1 diabetes 1 1 2,3 2 Mouna Stayoussef , Jihen Benmansour , Fayza A. Al-Jenaidi , Mansoor H. Rajab , 4 5 1 1 3 Hichem B. Said , Mohamed Ourtani , Chiheb B. Rayana , Touhami Mahjoub , Wassim Y. Almawi 1 Research unit of Hematological and Autoimmune Diseases, Faculty of Pharmacy, University of Monastir, Tunisia 2 Department of Pediatrics, Salmaniya Medical Complex, Manama, Bahrain 3 College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Bahrain 4 Department of Endocrinology, CHU Farhat Hached, Sousse 5 Hospital of Kairouan, Kairouan, Tunisia Correspondence: W.Y. Almawi, Ph.D., Department of Medical Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, P. O. Box 22979, Manama, Bahrain wassim@.bh Accepted for publication April 2, 2010 ABSTRACT. Aim . We investigated the association of tumor necrosis factor (TNF)α gene polymorphism with type 1 diabetes (T1D). Methods. TNF-α -1031T/C, -863C/A, -857C/T, -376G/A, -308G/A, -238G/A, and +488G/A single nucleotide polymorphisms (SNPs) were assessed in 198 T1DM patients and 180 age-and gender-matched, normoglycemic control subjects using PCR-restriction fragment leng