【2017年整理】RealTimeImagingofMitochondrialHydrogenPeroxideandpH.pptVIP

Real-Time Imaging of Mitochondrial Hydrogen Peroxide and pH Fluctuations in Living Cells Using a Fluorescent Nanosensor;回顾与背景介绍;;Cy-O-SeH;荧光素（fluorescein）;MSN;PEI;ZETA电位; TEM image of MSN (a) and nanosensor (b). (c) Sizedistribution of the prepared MSN. (d) Zeta potential of the every step modification: (1) MSN, (2) MSN-fluorescein-Cy-O-SeH, (3) MSN-fluorescein-Cy-O-SeH-PEI, (4) nanosensor.;(a) Fluorescence spectra of the nanosensor with various pH values from 5.3 to 8.2 (λex = 488 nm, 10 mM HEPES). (b) Linear fitting of the pH-dependent fluorescence intensity at 512 nm. (ΔF = F ? F 5.3 ). (c