雌激素与二甲肼对大鼠结肠上皮隐窝分裂的影响.pdfVIP

华 中 科 技 大 学 硕 士 学 位 论 文 Results ： No dividing crypt or ACF was observed in group A and C , both groups were treated without DMH. Crypt fission index in group D was larger than group B （17.60±4.93vs1.50±1.05%, P0.001 ） and E （17.60±4.93vs1.40±0.55%, P0.001）. The total number of ACF and the number of ACF with AC(6) in group D were larger than group B （ 57.80±6.54vs29.67±6.15 ， P0.001, 9.00±3.16vs2.83±1.33, P0.001 ） and E （ 57.80±6.54vs38.40±4.22 ， P0.001, 9.00±3.16vs4.40±1.34, P0.001 ）. The PCNA index in group D was much larger than group A(P0.001), B(P0.001), and C(P0.001) and smaller than group E(P0.001). The crypt volume increased exclusively in group D (P0.001). There was an evident correlations (P0.05) between the high crypt fission index and high amount of large ACF .. Conclusion ： DMH promoted crypt fission, while ovariectomy alone did not. However, ovariectomy could exaggerate while estrogen replacement inhibit the DMH induced elevation of crypt fission index. Crypt fission maybe correlated with the crypt volume but not with crypt proliferation. In addition, high crypt fission index may implicate the high risk of colon neoplasma because of a higher amount of large ACF. Keywords ：Crypt fission; ovariectomy; estrogen; DMH; colon cancer; ACF; cell proliferation.