雌激素对大鼠阴道平滑肌细胞RhoARho激酶表达的影响.pdfVIP

关系数r =0. 586 ，在0.01 水平（双侧）上显著相关）。OE4 和 OE6 组阴道平滑肌细胞Rock2 表达显著高于C4 和C6 组（0.377 ±0.035 vs 0.294 ±0.033 ；0.388 ±0.038 vs 0.300 ±0.034 ，P ＜0.05 ），经E2 替代4 和6 周达正常水平后，E4 和E6 组阴道平滑肌细胞Rock2 表达显著降 低至与对照组无显著差异，E2 水平与Rock2/ β-actin 吸光度比值呈显 著负相关（相关方程Y = 0.454 －0.003X，相关系数r =0. 729 ，在0.01 水平（双侧）上显著相关）。 结论：Rock1 、Rock2 主要表达在大鼠 阴道平滑肌细胞胞浆中，低雌激素水平上调Rock1 、Rock2 在大鼠阴 道平滑肌细胞中的表达，E2 替代治疗可抑制Rock1 、Rock2 在去势大 鼠阴道平滑肌细胞中的高表达，雌激素可能通过调控 Rock1 、Rock2 表达影响阴道平滑肌功能。 关键词：RhoA/Rho 激酶;雌激素;阴道;平滑肌 2 Effect of oestrogen on the expression of Rho-kinase in the vaginal smooth muscle cells of rats Abstract: Objective: To determine the expression of RhoA/Rho-kinase in the vaginal smooth muscle cells of castrated rats and the effect of estrogen on the expression of RhoA/Rho-kinase. Methods: 30 female SD rats of 10 weeks were randomly divided into groups C4(4 -week control group),C6(6-week control group),OE4(4-week bilateral ovariectomy),OE6(6-week bilateral ovariectomy),E4(4-week bilateral ovariectomy and oestrogen replacement) and E6(6-week bilateral ovariectomy and oestrogen replacement), Castrated and oestrogen replacement after one week adaptive feeding, intraperitoneal anesthesia with 1% Pentobarbital, control group rats were carried out incision lower abdomen without bilateral ovariectomy, the castrated group rats were carried out bilateral ovariectomy for 4 and 6 weeks , while Group E4 and E6 were carried out bilateral ovariectomy and oestrogen replacement for 4 and 6 weeks, feeding in 20 ℃-21 ℃, humidity 50%-65% environment. Four and six weeks after operation, determined the serum E2 level , the expressions of Rock1 and Rock2 in vaginal smooth muscle