凝胶渗透净化色谱-液相色谱串联质谱法测定化妆品中7种雌性激素.pdfVIP

日用化学工业 Vol. 43 No.5 第43 卷第5 期 2013 年 10 月 China Surlactant Detergent Cosmetics Oct. 2013 凝胶渗透净化色谱一液相包谱串联质谱法 测定化妆品中7 种雌性激素 陈溪l ，沈藻真1 ，张晓林l ，董伟峰2 ，刘 莹1 ，林维宣2 (1.大连出入境检验植霞周，辽宁 大连 1164∞;2. 辽宁出入境检验检疫局，辽宁 大连 116则} 摘要:建立了一种化妆品中雌性激素类药物的液相色谱-串联质谱的检测方法。对化妆品样品，采用乙睛超声提取，凝 胶渗透净化色谱(CPC) 净化，液质联用仪(LC - MS/MS) 分析。结果表明;7 种雌激素(雌二醇、雌三醇、雌酣、己皖雌盼、 己烯雌盼、己二烯雌盼和快雌醇)线性关系良好(r注0.995) j 方法检出限为O. 2 - 2. 4 mg . kg - 1 ，平均回收率为 69.4% - 108.7% ，相对标准偏差(RSD) 为4.5% -12.4% 。 关键词:化妆品;激素;凝胶渗透净化色谱;液质联用仪;检测 文章编号:1001-1803(2013)05 -0393-04 文献标识码:A 中图分类号:TQ658 Determination of seven kinds of estrogen in cosmetics by GPC -LC -MS/岛fS l l l 2 l 2 CHEN Xi ,SHEN Bao -zhen ,ZHANG Xiao -lin ,DONG Wei -feng ,LlUYing ，口N Wei -xuan ( 1. Dalian Entry - exit Inspection and Quarantine Bureau ,Dalian ,Liaoning 1164∞，Chinaj 2. Liaoning Entry - exit Inspection and Quarantine Bureau ,Dalian , Liaoning 116α泊， China) Abstract: A liquid chromatography - tandem mass spectrometry (LC - MS/MS) method was established for determination of seven kinds of estrogen in cosmetics. The cosmetic samples were extracted with acetonitrile under help of ultrasonic wave , and the extract was purified with gel permeation chromatography (GPC) , and then detected by LC - MS/MS. Results showed that the relation between peak area and mass concentration of the detected seven kinds of estrogen , including oestradiol , oestriol , oestrone , hexestrol , sti