凝胶渗透色谱-液相色谱-串联质谱法同时测定化妆品中15种糖皮质激素和性激素.pdfVIP

日用化学工业 Vol. 44 No.5 第44 卷第5 期 2014 年5 月 China Surtactant Detergent Cosmetics May 2014 凝胶渗透包谱 -1夜相包谱一串联质谱法同时测定 化妆品中 15 种糖皮质激素和性激素 崔晗，沈藻具，陈 溪，曹文军，刘梦遥，黄大亮 (大连出入境检验幢幢同，辽宁 大连 1164∞) 摘要:建立了一种凝胶渗透色谱-液相色谱-串联质谱法同时测定化妆品中 15 种糖皮质激素和性激素的方法。化妆品 样品采用乙腊超声提取，环己烧-乙酸乙醋体系的凝胶渗透色谱柱净化，以乙脯-体积分数为0.1% 甲酸水溶液作为流 R 均大于 色谱柱进行分离，由液相色谱-串联质谱仪定量检测。结果表明，方法的线性关系良好，相关系数 动相，经 C I8 0.995 ，方法检出限为0.1 -4.0 mg kg-I ，添加平均回收率为70.0% -104.0% ，相对标准偏差为 5.3% -15.5% 。 关键词:化妆品;糖皮质激素;性激素;凝胶渗透色谱;液相色谱-串联质谱 中固分类号:TQ658 文献标识码:A 文章编号:1001 -1803(2014)05 -0295-04 IHlI:I0.13218/j.cnki.csdc.2014.05.066 Simultaneous determination of 15 kinds of glucocorticoid and sex hormone in cosmetics by GPC - LC -岛fS/岛fS CUI Han ,SHEN Bao - zhen ,CHEN Xi ,CAO Wen - jun ,LIU Meng - yao ,HUANG Da -liang ( Dalian Entry - exit Inspection and Quarantine Bureau ,Dalian ,Liaoning 1164∞，China) Abstract: A method for the simultaneous determination of residue of 15 kinds of glucocorticoid and sex hormone in cosmetics by gel permeation chromatography - liquid chromatography - tandem mass spectrometη ( GPC - LC - MS/MS) was established. The sample was extracted with acetonitrile under ultrasonic assistance , and the extract was purifìed with GPC column of cyclohexane - ethyl acetate. Then the contents in the purified extract were separated by a CI8 column with acetonitrile -0.1% formic acid solution as the mobile phase ,and then detected by LC - MS/MS. Testing results showed that good linearity was obtained with correlation