原子力显微镜技术在多糖研究中应用.pdf

2008,41(10):3222-3228 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2008.10.040 1,2 3 1 1 1 1 1 2 3 430070 350108 430079 Application of Atomic Force Microscopy in the Study of Polysaccharide 1,2 3 1 1 1 1 WU Jia , DENG Xiao , ZHANG Yun , WANG Lan , TIAN Bin-qiang , XIE Bi-jun 1 2 ( College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070; College of Biological Science and Technology, Fuzhou University, Fuzhou 350108; 3 Wuhan Scientific Research Design Institute, State Administration for Grain Reservation, Wuhan 430079) Abstract: This paper gives a brief introduction to basic principle and operating mode of atomic force microscope (AFM). Sample preparation methods and the factors affecting the AFM image of polysaccharide are described in detail. Advance in using AFM for morphological observation and quantitative study on polysaccharide molecules are reviewed. Research work on single molecular mechanical spectroscopy of polysaccharide and determination and adjustment of conformational change of sugar residue are introduced. Prospects for the further application of AFM in polysaccharide investigation is presented. Key words: Atomic force microscope; Polysaccharide 1982 ·