PI3K通路介导apelin促H9c2大鼠心肌细胞肥大.pdf

目 录 一 主要英文缩略引„„„„„„„„„„„„„„„„1 二 中文摘要„„„„„„„„„„„„„„„„„„„2 三 英文摘要„„„„„„„„„„„„„„„„„„„4 四 论文正文 1 前言„„„„„„„„„„„„„„„„„„„„„6 2 实验材料„„„„„„„„„„„„„„„„„„„9 3 实验方法„„„„„„„„„„„„„„„„„„„11 4 实验结果„„„„„„„„„„„„„„„„„„„14 5 讨论„„„„„„„„„„„„„„„„„„„„„27 6 结论„„„„„„„„„„„„„„„„„„„„„34 五 参考文献„„„„„„„„„„„„„„„„„„„35 六 附录 成果目录„„„„„„„„„„„„„„„„„„„„4 1 发表论文„„„„„„„„„„„„„„„„„„„„42 文献综述„„„„„„„„„„„„„„„„„„„„60 文献翻译„„„„„„„„„„„„„„„„„„„„73 七 致谢„„„„„„„„„„„„„„„„„„„„„80 主要英文缩略语索引 Akt protein kinases B 又名PKB Apelin APJ endogenous ligand APJ 内源性配体 APJ putative receptor protein related to the angiotensin receptor AT 1 血管紧张素受体样受体或血管紧张素受体AT1 相关的受体蛋白 AngII Angiotensin II 血管紧张素II DMEM Dulbecco’s modified Eagle’s medium Dulbecc 极限必需培养基 DMSO dimethyl sulfoxide 二甲基亚砜 ERK1/2 extracellular signal-regulated kinase1/2 胞外信号调节激酶1/2 FBS fetal bovine serum 胎牛血清 GPCR G protein-coupled receptor G 蛋白偶联受体 MAPK mitogen-activated protein kinase 丝裂原激活蛋白激酶 MTT methylthiazoletrazolium 四噻唑蓝 oGPCR orphan G protein-coupled receptor 孤儿G 蛋白偶联受体 PAGE polyacrylamide gel electrophroesis 聚丙烯酰胺凝胶电泳 PI3K phosphatidylinositol-3-kinase 磷脂酰肌醇3 激酶 PMSF phenylmethylsulfonyl fluoride 苯甲基磺酰氟 PVDF Polyvinylidene Fluoride 聚偏二氟乙烯 SDS sodium dodecyl sulfate 十二烷基硫酸钠 VSMC vascular smooth muscle cell 血管平滑肌细胞 WB Western Blotting 蛋白质印迹 1 PI3K 通路介导apelin 促H9c2 大鼠心肌细胞肥大 摘要 目的： APJ 受体是孤儿G 蛋白偶联受体，apelin 是其内源性配体，本实验室已报道，在 双肾双夹易卒中型肾血管性高血压大鼠模型中apelin 表达异常增加并伴随心