白藜芦醇离体与在体对肝癌细胞Bel-7402的抑制作用及可能的效应机制分析.docVIP

白藜芦醇对肝癌细胞Bel-7402的抑制作用 及效应机制分析 [摘要] 目的 通过体内体外实验，观察白藜芦醇（resveratrol,Res）对肝癌细胞Bel-7402的增殖抑制作用，并对其抑癌的可能效应机制进行分析。方法设四个Res药物组最终浓度分别为12.5、25、50、100μmol/L，同时设置不含Res药物的对照组采用MTT法测试Res对体外培养肝癌Bel-7402细胞的增殖抑制作用，反转录PCR（reverse transcription-PCRRT-PCR）法检测Bcl-2 mRNA的表达，Western Blot法检测Bcl-2蛋白的表达，ELISA法测定Res对荷瘤小鼠细胞因子水平的影响。结果Res可以呈剂量和时间依赖性方式抑制肝癌Bel-7402细胞的增殖和Bcl-2 mRNA及其蛋白的表达。同时Res能明显抑制荷瘤小鼠肿瘤的生长，并能明显升高荷瘤小鼠体内细胞因子IL-2、IL-6、IL-12和TNF-α的水平。结论 Res与对肝癌细胞Bel-7402均有显著的增殖抑制活性，其抗肿瘤的效应机制可能与其下调Bcl-2的表达和提高细胞因子IL-2、IL-6、IL-12及TNF-α的水平有关。 [关键词] 白藜芦醇；肝癌细胞Bel-7402；抑制作用效应机制[中图分类号] [文献标识码] AThe inhibition and possible mechanism of resveratrol on hepatocellular carcinoma cell line bel-7402 both in vitro and vivo [Abstract] Objective To explore the inhibition effect and the possible mechanism of resveratrol on Human Hepatocellular Carcinoma Cell Line Bel-7402 Both in Vitro and Vivo. Methods Four Res drugs in the experiment group, the final concentrations were 12.5, 25, 50, 100 mol/L, the control group at the same time set not containing Res drugs,MTT assay was used to measure the inhibition of resveratrol on Bel-7402. The expression of Bcl-2 was detected by RT-PCR and Western Blot. The levels of IL-2、IL-6、IL-12 and TNF-αwere detected by ELISA. Results Resveratrol inhibited Bel-7402 cell proliferation in dose and time manner, and influence the expression of Bcl-2. At the same time, Resveratrol can inhibit the growth of tumor and improve the levels of IL-2、IL-6、IL-12 and TNF-α.Conclusion Resveratrol inhibited Bel-7402 cell proliferation both in vitro and vivo, the possible mechanism may be that resveratrol can cut the expression of Bcl-2 and improve the levels of IL-2、IL-6、IL-12 and TNF-α. [Keywords] Resveratrol; Hepatocellular Carcinoma Cell Line Bel-7402; inhibition; Mechanism 肝癌是一种十分常见的恶性消化道肿瘤，其病死率高、危害性大，已经位居世界肿瘤性疾病的第3位，且有逐年升高的趋势[1-3]。中国是肝癌的高发区，其发病人数约占全世界总发病数的一半，而其死亡率也位居世界之首[4]。目前，治疗肝癌的首选方法是进行手术切除，约有40%的肝癌患者均采用手术切除治疗[5]。近年来，随着外科手术技术的快速发展，肝癌的手术治疗效果也在一定程度上得到改善。但是，肝癌术后复发率相当高，5年后复发率高达80%左右，患者的总体生存率并未得到明显提高