以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究论文.docVIP

　　以高通量方法筛选红桑叶的抗菌活性并进行活性组分的分离研究论文 【摘要】 目的通过一种简单快速的高通量方法筛选具有抗菌活性的植物提取物，并通过此种方法从有活性的粗提物中纯化活性组分。 方法首先建立植物提取物库和抗菌的高通量筛选方法。然后针对转糖链球菌Streptococcus mutans和伴放线放线杆菌Actinobacillus actinomycetemitans进行高通量筛选。 结果通过筛选发现红桑叶具有很强的抑菌活性。为了进一步研究其活性，将其粗提物通过HPLC进行分离得到71个组分，再通过同样的高通量筛选方法进行抗菌活性测定，对有活性的组分通过血琼脂法进行验证。实验数据表明红桑叶的一个组分#31对两种细菌有几乎完全的抑制（抑制率 99%），而另外两个组分对伴放线放线杆菌Actinobacillus actinomycetemitans有部分抑制。结论 所建立的高通量筛选方法简便快捷，筛选结果稳定可靠，可用于大规模的植物提取物的抗菌活性筛选。红桑叶具有很强的抗菌活性.freelple and fast colorimetric detection method and identify those plant extracts icrobial High Throughput Screening (HTS) method. Then, the plant extracts utans and A. actinomycestemitans. ResultsThe Morus rubra leaf extract all fractions via high-performance liquid chromatography (HPLC). The fractions utans and A. actinomycestemitans using HTS and the antibacterial activity of those selected bioactive fractions ed by blood agar plating method. One fraction of Morus rubra leaves, #31, exhibited near plete inhibiton (99% or better) for both bacteria, e, but not plete, inhibition of A. actinomycestemitans. ConclusionThe HTS can be used to screen for a large plant extract library due to its fast and simple procedure. Morus rubra leaves exhibited the strongest antibacterial activity and the active fraction portant alternatives to the traditional antibiotics to bat the recent development of antibiotics-resistant bacterial strains due to the overuse of over-the-counter antibiotics ［1，2］. Plant materials and plant parts have been used as medicine in China to treat diseases for centuries. Plants are the rich source for drug discovery due to the secondary metabolites produced. Plant-based antibiotics products can serve as an important source for future therapeutic treatment of infectious diseases ［3，4］. Screening for antibiotics from a vast plant source can be a daunting task because there are hundreds of plant species and hundreds of plant secondary metabolites even ethods to identify antibacterial phytochemicals from a plant extra