锌对铁胁迫下小麦幼苗根抗坏血酸过氧化物酶和谷胱甘肽还原酶活性的影响.doc

目 录 摘要......................................................................................................................................2 1 前言................................................................................................................................2 2 材料方法........................................................................................................................4 2.1 植物材料的培养......................................................................................................4 2.2 酶活检测..................................................................................................................4 2.2.1 APX活性的检测................................................................................................4 2.2.2 GR活性的检测...................................................................................................4 2.2.3 蛋白含量的检测数据分析抗坏血酸过氧化物酶谷胱甘肽还原酶 XXX （西北师范大学 生命科学学院，甘肃，兰州，730070） 摘要：μmol · L-1、250 μmol · L-1及2 mmol · L-1)对300 μmol · L-1Fe胁迫下小麦幼苗根中APX和GR活性的变化。实验发现，单独Fe胁迫诱导了小麦幼苗根系中APX活性的升高和GR活性的降低。与单独Fe处理相比，50 μmol · L-1 Zn的加入降低了小麦根APX活性，而250 μmol · L-1和2 mmol · L-1Zn的加入明显增加了该酶的活性；不同浓度Zn的使用均明显增加了单独Fe处理下小麦幼苗根GR的活性。结果表明，Zn的加入诱导Fe胁迫下小麦幼苗根中APX和GR活性的升高，有助于缓解Fe对植物根的毒害作用。 关键词：锌； Effect of Zn on ascorbate peroxidase and glutathione reductase activities in wheat seedling roots under Fe stress XXX （College of Life ScienceNorthwest Normal University, Lanzhou 730070, Gansu, China） Abstract：The study aimed to investigate the effects of different Zinc concentrations (50 μmol · L-1、250 μmol · L-12 mmol · L-1) on the activies of APX and GR in Xihan wheat seedlings exposed to 300 μmol · L-1 iron(Fe) treatment. The results showed that single Fe stress induced the increase of APX activity and the decrease of GR activity in wheat seedling roots．Compared with the single Fe stress, the addition of 50 μmol · L-1 Zn reduced APX activity, while 250 μmol · L-1和2 mmol · L-1Zn applied enhanced the activity of this enzyme in wheat roots. The appl