植物乳酸菌广谱抑菌成分特性的研究.pdfVIP

摘 要 植物乳酸菌能够产生广谱抑菌物质，能较强的抑制革兰氏阳性菌枯草芽孢杆 菌和金黄色葡萄球菌、革兰氏阴性菌大肠杆菌和鸡白痢沙门氏菌及真菌酵母菌。 通过单因素试验和正交试验优化植物乳酸菌产抑菌物质的培养条件，以便获得最 大量的抑菌物质，并探索抑菌物质的提取和其特性。实验结果表明： (1) 菌株生长的最佳培养条件组合是温度 30℃，培养基初始pH 为 6.0，接 种量2%，种龄22 h, 厌氧条件下，培养时间40 h。植物乳酸菌产抑菌物质对革 兰氏阴性菌的抑菌作用最大的最佳培养条件组合是温度28 ℃，培养基初始pH 为 7.0，接种量2%，种龄20 h, 厌氧条件下，培养时间24 h。植物乳酸菌产抑菌物 质对革兰氏阳性菌的抑菌作用最大的最佳培养条件组合是温度 28 ℃，培养基初 始pH 为6.5，接种量1%，种龄16 h, 厌氧条件下，培养时间40 h。 (2) 发酵液上清液中的抑菌物质非过氧化氢和乳酸，并且对胃蛋白酶、胰蛋 白酶以及木瓜蛋白酶不敏感。此抑菌物质有很强的热稳定性，能够耐受121℃20 min ，能在小于4.5 的pH 范围起作用。 (3) 发酵液能够通过透析（MWCO 为1000）和AB-8 和DM101 大孔树脂层 析初步分离。该实验菌株的抑菌成分为分子量小于1000 的极性小分子。 关键词：植物乳酸菌；抑菌物质；发酵条件；杯碟法 I Study on Characterization of Antibacterial Substance from Lactobacillus Plantrum Abstract The Lact obacterillus Plantrum can produce broad-spectrum antimicrobial substance, which inhibitted strongly gram-positive bacteria such as Bacillus subtilis and Staphylococcus aureus, Gram-negative bacteria such as Escherichia coli and Salmonella pullorum and yeast fungi. In order to maximize the yield of inhibitory material by liquid cultivation of Strain of Lactic Acid bacterium, Single- factor-experiment design and orthogonal experiment design were used to optimize the culture conditions.The purification and characterization of this substance were also studied. The results as follows: (1) The optimal conditions for its growth were obtained at culture time of 40 h, 30℃，initial pH 6.0, inoculation rate of 2%and seed age of 12 h under anaerobic condition. Following fermentation conditions for the highest inhibition activity to gram-negative bacteria were with culture