大豆异黄酮对奶牛肠系淋巴结与脾脏淋巴细胞白介素2、干扰.docVIP

大豆异黄酮对奶牛肠系淋巴结和脾脏淋巴细胞白介素2、干扰素γ雌激素受体βmRNA表达的影响 王明1孙志刚1刘文奇2杨柳1方洛云1蒋林树1*( （1北京农学院动物科技学院反刍动物营养实验室，北京102206；2北京首都农业集团有限公司，北京100029） 摘要：本试验旨在研究大豆异黄酮对奶牛脾脏与肠系淋巴结淋巴细胞增殖、活化及细胞因子水平的影响。采用试验设计，浓度100.00μg/mL]与淋巴细胞共育4、24和48h，用法测定培养上清液中的白介素2（IL-2）、白介素4（IL-4）、干扰素γ（IFN-γ），用RT-PCR方法测定淋巴细胞中ER-β）mRNA表达量。结果表明：1.00和0.25μg/mL浓度显著提高脾脏淋巴细胞中IL-2和IFN-γ水平（P0.05），而25.00μg/mL SI能显著抑制脾脏淋巴细胞IL-2和IFN-γ水平（P0.05）。5.00和1.00μg/mL能显著提高肠系膜淋巴结中淋巴细胞IL-2和IFN-γ水平（P0.05）。各组均能降低淋巴细胞培养上清液中IL-4水平，提高IFN-γ/IL-4的比值。各大豆异黄酮添加组均能显著或极显著提高奶牛脾脏和肠系膜淋巴结中淋巴细胞雌激素受体β（ER-β）mRNA的表达，且存在着正比的剂量关系。 关键词：大豆异黄酮；白介素2；干扰素γ；雌激素受体β；免疫Effects of Soy Isoflavones on The Levels of Interleukin-2，Interferon-gamaand The Expression of Estrogen Receptors-β mRNA in Lymphocyte of Lymph Nodes and Splenocytes of Dairy Cow WANG Ming1 SUN Zhigang1 Liu Wenqi2YANG Liu1Fang Luoyun1JIANG Linshu1*( （1. College of Animal Sciences, Laboratory of Molecular Animal Nutrition, Beijing University of Agriculture, Beijing 102206,China;2. Beijing Capital Agribusiness Group, Beijing 100029,China） Abstact: Soy isoflavones are a group of plant-derived biologically active substances with a chemical structure that resembles that of 17β-estradiol(E2).As the presence of estrogen receptors(ER)has been identified in several immune cells, soy isoflavones may also have a great impact on the immune system. This paper aims to research the in vitro effects of soy isoflavones on the proliferation and activation and the production of cytokines by stimulated lymphocyte of lymph nodes and splenocytes from dairy cow, investigate to improve the mechanism of immune of cows,toprovide a theoretical basis of the soy isoflavones developed into a functional feed additives. The experiments were assigned into 6 groups(blank,100.00,25.00,5.00,1.00,0.25μg/mL Soy isoflavones) and incubated with lymphocyte to 4、24 or48h,respectively. The levels of interleukin-2(IL-2)、interleukin-4(IL-4)and interferon-γ(IFNγ) in culture supernatants were determined by ELISA, and ER-β mRNA were determined by reverse t