金黄色葡萄球菌SEG蛋白B细胞线性表位的预测及验证.PDFVIP

安徽农业大学学报, 2013, 40(3): 426-429 Journal of Anhui Agricultural University 网络出版时间：2013-4-26 15:37:02 [URL] /kcms/detail/34.1162.S1537.016.html 金黄色葡萄球菌 SEG 蛋白 B 细胞线性表位的预测与验证 * 梁明燕，戚莉芳，李槿年 ，张婷婷 (安徽农业大学动物科技学院，合肥 230036) 摘 要：以金黄色葡萄球菌标准株ATCC25923 的肠毒素G 蛋白（SEG ）氨基酸序列为分析材料，应用DNAstar 软件对该蛋白的二级结构、柔性区域、亲水性、表面可能性和抗原指数等参数进行综合分析，预测出 SEG 蛋白 5 个可能的B 细胞线性表位，它们分别位于蛋白N 端第24 ～31、37 ～46 、80 ～84、120 ～127 和 141 ～149 区域，且 预测的表位2 （aa 37 ～46 ）、表位4 （aa120 ～127 ）和表位5 （aa141 ～149 ）位于SEG 蛋白三维结构表面。合成位 于蛋白三维结构表面的3 个预测表位，采用肽ELISA 方法加以鉴定。结果表明，它们均能与抗重组SEG 蛋白纯化 抗体发生特异性结合反应，是SEG 蛋白的B 细胞线性表位。 关键词：金黄色葡萄球菌；SEG 蛋白；B 细胞线性表位；预测；肽ELISA 中图分类号：S852.61 文献标识码：A 文章编号：1672−352X (2013)03−0426−04 Prediction and verification of the linear B-cell epitopes of staphylococcal enterotoxin G protein Liang Ming-yan ，QI Li-fang，Li Jin-nian ，ZHANG Ting-ting (School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036) Abstract: In order to locate the B-cell linear epitopes of SEG protein, epitope prediction was performed us- ing the amino acid sequence of SEG protein from Staphylococcus aureus standard strain ATCC25923. The secon- dary structure, flexible regions, hydrophilicity, surface accessibility and antigenic index of the protein were ana- lyzed using the DNAStar Protean program, and five potential epitopes were screened, which were located at amino acid positions 24 to 31, 37 to 46, 80 to 84,120 to 127 and 141 to 149. Among them, the epitopes at amino acid positions 37 to 46, 120 to 127 and 141 to 149 were entirely exposed on the surface of the 3D structure model of SEG protein. To verify the prediction, three potential epitope peptides exposed on the surface of the 3D struc-