杧果种质遗传多样性的表型分析和AFLP分析.pdfVIP

园 艺 学 报 2011 ，38 （3 ）：449 –456 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 杧果种质遗传多样性的表型分析和 AFLP 分析 石胜友，武红霞，王松标，刘丽琴，王一承，马蔚红* （中国热带农业科学院南亚热带作物研究所，广东湛江 524091 ） 摘 要：利用表型和 AFLP 标记，对中国热带农业科学院南亚热带作物研究所杧果种质资源圃的 56 份国内外杧果种质进行遗传多样性分析。结果表明：这些种质的表型性状表现出较大的差异，多样性指 数（H ′）在0.56 ~ 1.64 之间，平均为 1.05，表型性状欧氏距离系数在 0.02 ~ 0.45 之间。8 对 AFLP 引物共 扩增出 713 条谱带，其中多态性带为 630 条，占 88.36%，遗传相似性系数 0.38 ~ 0.85 之间。Mantal 测量 结果表明表型和基因型距离矩阵间存在显著的正相关（r = 0.72 ，P = 0.05 ）。表型性状和AFLP 分子标记 分析的结果相对一致，56 份杧果种质具有较丰富的遗传多样性。 关键词：杧果；AFLP 标记；表型性状；遗传多样性 中图分类号：S 667.7 文献标识码：A 文章编号：0513-353X （2011 ）03-0449-08 Genetic Diversity of Mango Germplasm Based on Morphological Charaters and AFLP Markers * SHI Sheng-you，WU Hong-xia ，WANG Song-biao ，LIU Li-qin ，WANG Yi-cheng ，and MA Wei-hong （South Subtropical Crops Research Institute ，Chinese Academy of Tropical Agricultural Sciences，Zhanjiang ，Guangdong 524091，China） Abstract ：The genetic diversity of 56 accessions of mango germplasm，which were maintained in South Subtropical Crops Research Institute ，Chinese Academy of Tropical Agricultural Sciences，China， were analyzed using morphological characters and AFLP markers. The results showed that these mango germplasm have rich genetic variation in morphology. The Shannon-Weinner index ranged from 0.56 to 1.64，and the Euclid distance of the morphological characters analyze ranged from 0.02 to 0.45. A total of 713 fragments were generated from 8 pairs of selective AFLP primer combination，630 （88.36% ）of which were polymorphic. The molecular genetic similarity coefficients ranged from 0.38 to 0.85. The Mental test showed that phenotypic variation was obviously positively correlated with the gene