过氧化氢催化荧光法测定维生素c大学论文.docVIP

化学工程与现代材料学院201届本科毕业论文 过氧化氢催化荧光法测定维生素C 化学专业 张满强 指导教师 刘萍 摘要：维生素C也称抗坏血酸，它是动物和人体生命活动必须的一种物质，当机体从外界摄取的维生素C不能满足其生命活动的需要或摄入的量超过人体吸收的最大能力，就会引起新陈代谢功能的紊乱甚至死亡。因此探究一种简单、灵灵敏的、快速的维生素C测定方法具有重要意义。 本论文利用在PH为6.8的磷酸介质中（由氢氧化钠和磷酸二氢钾配置），70℃水浴加热12min，维生素C对过氧化氢氧化二氯荧光素的反应具有显著地催化作用，由此建立了催化动力学荧光法测定维生素C的新方法。探讨了该催化反应的较优试验条件并测定了动力学参数，在最佳条件下，非催化体系荧光强度F与催化体系荧光强度F之间的差值△F与维生素C浓度在0.13～mg/L范围内呈良好的线性关系，方法的检出限为0.78 mg/L，反应为零级反应，表观速率常数为3.5×10-4 mol/L·s-1。表观活化能为Ea = 47.31 kJ/mol。该方法灵敏度高、仪器简单。 关键词：催化荧光法；过氧化氢；二氯荧光素；维生素CDetermination of vitamin C by hydrogen peroxide catalytic fluorimetry Chemistry Specility ZhangManqiang Tutor LiuPing Abstract:Vitamin C also known as ascorbic acid, it is the activities of animal and human life must be a substance, when the body from outside the uptake of vitamin C can not meet the need or intake amount exceeds the human body absorption maximum capacity, it will cause metabolic disorders and even death. Therefore, to explore a simple, sensitive, rapid Lingling vitamin C determination method has important significance. At pH 6.8 phosphate medium (by sodium hydroxide and potassium dihydrogen phosphate configuration), 70℃ water bath heating 12min, the reaction of vitamin C on the hydrogen peroxide oxidation of dichlorofluorescein has significant catalytic effect, which resulted in the establishment of the Catalytic Kinetic Spectrofluorimetric Determination of Vitamin vitamin C of the new method by this thesis. Discusses the optimized experimental conditions for the catalytic reaction and kinetic parameters were determined. Under the optimum conditions, the non catalytic fluorescence intensity of the system f and catalytic fluorescence intensity of the system f between difference delta f and the concentration of vitamin C in 0 ~ 9.024 mg / L﹡range showed a good linear relationship, the method detection limit was 0.78 mg / L, and the reaction is zero order reaction, the apparent rate