桑椹片中多酚及花青素含量的测定.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.4 桑椹片中多酚及花青素含量的测定 林耀盛，刘学铭，杨荣玲，陈智毅，杨春英，赵晓丽，王思远 （广东省农业科学院蚕业与农产品加工研究所，省部共建国家重点实验室培育基地-广东省农产品加工重点实验 室，广东广州 510610） 摘要：采用Folin-Ciocaiteu 法测定桑椹片中总多酚含量，通过HPLC 法分析其中花青素的主要花青素单体及含量，及运用pH 示 差法测定其总花青素含量。该桑椹片的总多酚含量63.76 mg GAE/g，总花青素含量为6.63 mg/g；从HPLC 花青素谱图中主要检测到 矢车菊素-3- 葡萄糖苷、矢车菊素-3-芸香苷这两种单体，其含量分别为2.91 mg/g 和2.55 mg/g，含量之比1.14:1。该方法在2~500 µg/mL 有很好的线性关系 1.0000~0.9999，平均回收率 102.5~97.8%，RSD 为0.93~1.01%。研究结果表明，该方法更为灵敏、准确、重现性 好，通过HPLC 方法建立，综合了多酚和花青素单体的含量及其比例的测定能够有效地对桑椹片的质量控制。 关键词：桑椹片；花青素；HPLC ；质量控制 文章篇号：1673-9078(2013)4-890-893 Determination of Polyphenols and Anthocyanins Contents in Mulberry Tablet LIN Yao-sheng, LIU Xue-ming, YANG Rong-ling, CHEN zhi-yi, YANG Chun-ying, ZHAO Xiao-li, WANG Si-yuan (The Sericulture Agri-Food Research Institute of Guangdong Academy of Agricultural Scienc es, Guangzhou 510610, China) Abstract: The Folin-Ciocaiteu method was used to determine the total polyphenol content of mulberry tablet. The main monomer and contents of anthocyanins were determined by HPLC. The pH differential method was applied to determine the total anthocyanins content. The total polyphenol content of Lipid-lowering tablets was 63.76 mg GAE/g and the total anthocyanin content was 6.63 mg/g. The monomer of cornflower-3-glucoside and cornflower-3-rutin were the main anthocy anin compounds with thire contents being of 2.91 mg/g and 2.55 mg/g, respectively. And the ratio of the two compounds was 1.14:1. A better linear relation was found in the range of 2~500 µg/mL 2 (R =1.0000~0.9999) and the average recovery rate was in the range of 102 .5~97.8% with the RSD of 0.93~1.01%. The method applied in this research was sensitive,