高效液相色谱法对蓝莓果实中8个有机酸含量测定_徐玉涛.pdfVIP

※成分分析 食品科学 2015, Vol.36, No.18 127 高效液相色谱法对蓝莓果实中8 个 有机酸含量的测定 1 1 2 3 1,* 徐玉涛 ，李珂珂，王贺新，陈丽荣，弓晓杰 （1.大连大学医学院，辽宁 大连 116622 ；2.大连大学现代化农业研究院，辽宁 大连 116622 ； 3.大连大学环境与化学工程学院，辽宁 大连 116622 ） 摘 要：建立同时测定蓝莓果实中8 个有机酸类成分含量的高效液相色谱法，并利用该方法测定26 个不同品种蓝莓 果实中的有机酸含量。结果表明，所测品种中塞拉的有机酸含量最高，达25.135 26 mg/g鲜果，最低的为克瑞顿， 仅含2.368 31 mg/g鲜果。该方法简便、快速、准确，具有良好的重复性和回收率，可作为蓝莓果实中有机酸含量的 测定方法。 关键词：蓝莓；有机酸；柠檬酸；高效液相色谱；含量测定 Determination of Eight Organic Acids in Blueberries by HPLC 1 1 2 3 1,* XU Yutao , LI Keke , WANG Hexin , CHEN Lirong , GONG Xiaojie (1. College of Medical, Dalian University, Dalian 116622, China; 2. Modernization of Agricultural Research Center, Dalian University, Dalian 116622, China; 3. College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China) Abstract: A high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 8 organic acids in blueberry fruit extract. This method was then used to determine the contents of organic acids in blueberries from 26 cultivars. Among the blueberry cultivars tested, the content of total organic acids (25.135 26 mg/g fresh fruit) was highest in Sierra and lowest in Croaton (only 2.368 31 mg/g fresh fruit). This method is simple, reproducible, accurate, reliable and useful for the analysis of organic acids in blueberries. Key words: blueberry; organic acid; citric acid; high performance liquid chromatography (HPLC); quantification 中图分类号：O656.3 ；R917               文献标志码：A 文章编号：1002-6630 （2015 ）18-0127-05 doi:10.7506