活化基因序列串联拷贝数目对GFP报告基因表达的影响及核心序列分析.pdf

序列分析 引言···……···…······…······…·········……………···………······…l5 第一部分SV40PolyA片段串联拷贝数目影响GFP报告基因表达 前言······………·············……·····……………······……············20日IJ舌一‘”‘”。。””。”’”’’。’‘。。。……’’‘‘‘·””……‘”‘”’’“”’“。‘。。‘”“‘‘”20 OO OOOO O O 90 O 0…………OIOOO00 材料与方法………O 0………………………20 结果············…··················…············…………··················29 附图、附表……………………………………………………………33 讨论············································-·············……··············48 0000 ／J、结············0 0…············…················……··········一…·一··51 参考文献………………………………………………………………51 第二部分PolyA反序活化基因片段碱基突变对GFP基因表达的影响 前言…………………………………………………···…···……···…54刚舌“一”””“”一”…”一””……一””””““”“”一”一””一”…”一”54 材料与方法……………………………………………………………54 结果··…··········…··············…···…············…··…·······54 oo 附图、附表…………………………eooe……………………………57 O OOO O 0 讨论··················…·············-················OO OOO 0 000·····…····61 OO0000OO·…········00·00 小结···O 0·-··············································62 结论···················…···············································……···63 综述一Line．1和Alu重复序列对基因表达的影响及在肿瘤发生中的作 用·······一·…······”…一””······”··”·············””··”··········…··64 综述二茎环结构形成的体外研究………………………………………78 致谢…··········…·········-····…························…·············…···········92 00o…………………………………93 个人简历……………………………0·o 达， 来自父方和母方的抗原受体等位基因在成熟的淋巴细胞中只有一个表现 活性。B淋巴细胞单等位基因表达抗原受体(BCR)是通过沉默两个同源 抗原受体之一完成，如果没有等位基因排斥，相同的B细胞将产生两个 重链和轻链独立互相结合，可以产生30种不同的异4聚体，不利于抗体 对抗原的识别，而等位基因排斥保证表达有效的多价抗体分子，保证一个 B细胞仅产生一种BCR。等位基因排斥似乎是一种普遍的表观遗传现象， 嗅神经受体等位基因排斥保证嗅觉识别的精