紫外法快速测定中原地区12种蔬菜VC含量.docVIP

紫外法快速测定中原地区12种蔬菜VC含量 　　摘要：以中原地区12种常见蔬菜为原料，采用紫外分光光度法对蔬菜维生素（VC）含量进行了测定。结果表明，pH=6时，维生素C最大吸收波长为265 nm，标准曲线方程A=0.048 61C-0.000 96，R2为0.998 8。12种中原蔬菜VC含量范围为0.063 5～2.718 7 mg/g，其含量高低顺序为芫荽青椒菠菜生菜上海青葱白菜长梗白菜白萝卜胡萝卜芹菜油麦菜。其中，芫荽VC含量最高，为2.718 7 mg/g；其次是青椒和菠菜，分别为2.101 7 mg/g和1.820 4 mg/g；油麦菜VC含量最少，仅为0.063 5 mg/g。芫荽、青椒、菠菜等蔬菜由于VC含量较高，有望成为VC补充的重要食源及VC功能产品研发的重要原料 关键词：中原地区；蔬菜；维生素C；紫外分光光度法 中图分类号：R151.3 文献标识码：A 文章编号：0439-8114（2017）06-1131-03 DOI：10.14088/j.cnki.issn0439-8114.2017.06.035 Abstract： The VC content of 12 kinds of winter vegetables from the central plains region was determined by ultraviolet spectrophotometry method. The results showed that the maximum absorption wavelength was 265 nm when pH=6. Standard curve equation was A=0.048 61C-0.000 96，R2=0.998 8. The content of VC in vegetables was from 0.063 5 mg/g to 2.718 7 mg/g. The content order was Coriandrum sativum L.Capsicum annuumSpinacia oleracea L.Lactuca sativaBrassica chinensis L.Allium fistulosum L. Brassica pekinensis RuprBrassica rapa chinensisRaphanus sativus L.Daucus carotaApium graveoleus L. var. dulce DC.Lactuea sative var longifoliaf Lam. The content of VC in Coriandrum sativum L. was much higher than other vegetables，up to 2.718 7 mg/g；Secondly，Capsicum annuum and Spinacia oleracea L. wasrespectively 2.101 7 mg/g and 1.820 4 mg/g；VC content in Lactuea sative var longifoliaf Lam. was the lowest，only 0.063 5 mg/g；Coriandrum sativum L，Capsicum annuum and Spinacia oleracea L. with high VC content were expected to become important food sources for VC supplement， and research and development of VC function product. Key words： central plains； vegetables； Vitamin C； UV spectrophotometry 维生素C（Vitamin C，简称VC）别名抗坏血酸，是人体必需的营养物质之一，参与体内氨基酸与神经传递、胶原蛋白合成，对体内某些代谢反应起到辅助作用，并可以促进钙、铁的吸收，因而对人体生理健康异常重要。医学研究结果表明，VC具有治疗坏血病、美白养颜、提高免疫力、预防感冒等功效，特别在治疗肝炎、肝硬化等方面能产生有益的疗效[1，2]。新鲜的蔬菜和水果中VC含量较为丰富，是人们进行VC补给的重要食源。据食品营养学记载[3]，蔬菜中的VC受地形、气候、土壤及光照时间的影?较为显著，即使同一蔬菜，其VC含量也会因产地、产季、生产条件不同而不同。中原地区属亚热带季风气候，春秋季节晴朗日照充分，夏季雨水丰沛，利于蔬菜的栽培生长。开展中原蔬菜VC含