第九章 高效液相色谱-串联质谱法分析.pdf

39 ( FENXI H UAXUE) 4 20 11 4 Chinese Journal of Analyti al Chemistry 534~ 539 DOI: 10. 3724/ SP. J. 1096. 2011. 00534 1, 2 * 1 3 3 3 1 1 ( , 214122) 2 3 ( , 2 14 122) ( , 214002) ( LCMS/ M S) E. coli 5 , / 3 , 3 , , ( RSD 5% ) 5 , : / , , , , 0. 05~ 0. 36 mmol/ L ; ; ; 1 , , , , , , [ 1] [ 2] , Escherichia coli ( E. coli) 3% ~ 5% , [ 3] [ 4~ 6] , ( GC MS) [ 7] , E. coli , / , LCM S/ M S ( M RM) , , 3 8 ( 24 ) , 5 , 2 2. 1 1200 ( Agilent ) ; 3200 Q TRA P ( Applied Biosystems ) , ; 3 L ( NBS) ; ( T ermo ) , ( NBS ) ; ( Lab on o ) ; JY2006 ( Mettler T pledo ) ; YDS6 ( ) 2BBI , ; ( No. SKLFT S200803) * Email: dj998l @ yahoo. om. n 4 : 535 Sigma ; ( , Tedia ) ; Millipore 2. 2 E. coli BW25113 ( DE( trp R, tna A, phe A) ) , 4 2 4 2 4 : 3. 0 g/ L M gSO 7H O, 5. 0 g/ L