对于氨基酸及神经肽及疼痛关系的研究.pptVIP

Background 背景 Cerebral spinal fluid (CSF) reflects metabolic state of brain 脑脊液反映疼痛的新陈代谢状态 Analysis of CSF amino acids and peptides – highly relevant in pain perception and also neurological diseases 脑脊液中氨基酸和缩氨酸与疼痛感知和神经系统疾病高度相关 Chronic Pain – inflammatory, neuropathy and cancer 慢性疼痛分类—发炎，神经系病，癌症 Mechanisms very challenging and not yet understood 对于疼痛机制，目前还没有被足够的了解，非常具有挑战性 Lack of effective therapeutics 缺乏有效的治疗法 Background Excitatory and inhibitory amino acids and also nitric oxide (NO) are involved in pain modulation. 兴奋性，抑制性氨基酸和一氧化氮涉及到对疼痛的调节 Neuropeptides are implicated in pain modulation. 神经肽影响对疼痛的调节 Objectives A two pronged study on CSF 对脑脊液的两次研究 To quantitatively analyze physiological amino acids and pain related compounds in CSF samples from pain patients 对疼痛病人脑脊液中生理氨基酸和疼痛相关的化合物定量研究 To find new pain-related peptides (molecular mass 15,000 Da) from CSF samples of chronic pain patients 从慢性疼痛病人的脑脊液中寻找与疼痛相关的肽（分子量小于15000） Results should lead to better understanding of pain mechanisms and new treatment strategy 实验结果帮助我们更深的了解疼痛机制和寻找新的治疗方法 Amino acids in CSF and Pain脑脊液中氨基酸和疼痛关系的研究 Typical Chromatogram典型色谱层析图 Salient features of the method 方法特色 Use of Triton X-100 （ Triton X-100 的应用） No pre-treatment of CSF 脑脊液不被预处理 Enhanced recovery of hydrophilic amino acids 提高亲水性氨基酸的恢复 Detection of citrulline improved 提高对瓜氨酸的检测 Requires small volume of CSF - 10-20 ?L 使用较少量的脑脊液 20 ?L of CSF sample yields 140 ?L of dabsylated sample. 20微升脑脊液样本经过dabsylation产生140微升样本 Injection volume – 20 ?L 一次样本液相色谱分析只需20微升 6 repeated analysis of same sample once dabsylated is possible 能够对同一 Dabsylate 过的样本进行6次重复分析 Q effectively separated - 100 times higher concentration in CSF 谷酰胺被有效分离—在脑脊液中浓度是其它氨基酸的100倍 Stability of dabsyl derivates Dabsyl衍生物稳定性 Dabsylated sample stable for one week in 4?C and for 48 hrs at room temperature 经过Dabsylation的样本能够在4度保持一周，室温下保持