甲壳素降解液防治番茄南方根结线虫病.pdfVIP

中国蔬菜 2010（16）：58-61 CHINA VEGETABLES 甲壳素降解液防治番茄南方根结线虫病 * 张志红 冯 宏 李华兴 张新明 袁英英 （华南农业大学资源环境学院，广东广州 510642） 摘 要：以番茄为试材，通过盆栽试验，研究 4 株芽孢杆菌与 1 株酵母菌组合后对甲壳素的降解效果， 以及甲壳素降解液防治番茄南方根结线虫病的效果。结果表明，所有菌株组合后的降解效果最好，其降解 液的粘度最低、几丁质酶活性最高。用甲壳素降解液处理番茄根际土壤，可提高土壤中细菌、真菌和放线 菌的群落数量，降低番茄南方根结线虫的根结率和根结指数。甲壳素降解液对南方根结线虫病防治效果为 54.29 %，是甲壳素培养基处理的 2.58 倍。 关键词：芽孢杆菌；酵母菌；甲壳素降解液；番茄南方根结线虫病 中图分类号：S436.412 文献标识码：A 文章编号：1000-6346（2010）16-0058-04 Control Effect of Chitin Degradation Liquid on Tomato Root-knot Nematodes * ZHANG Zhi-hong, FENG Hong, LI Hua-xing , ZHANG Xin-ming, YUAN Ying-ying （College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, Guangdong, China ） Abstract ：This paper mainly studied chitin degradation effect of different combination of several microbes（Bacillus spp. and Saccharomyces spp.）and control effect of chitin degradation liquid（CDL） on tomato（Lycopersicum esculentum Mill.）disease of root-knot nematodes by artificially inoculating Meloidogyne incognita . The results indicated that mixture of 4 Bacillus strains and a Saccharomyces strain had best degradation effect on chitin with the lowest viscosity, the highest chitinase activity. CDL could control tomato root-knot nematodes disease effectively, decrease root-knot rate and root-knot index. Therefore，control effect was 54.29 %, which was 2.58 times compared with the treatment of chitin liqiud medium. CDL could also improve the populations of bacteria, fungi and actinomycetes in soil. Key words ：Bacillus; Saccharomyces; Chitin degrad