lishufeng-高级生化实验.pptVIP

2. In terms of solubility isoelectric precipitation Salting in and salting out Organic solvent precipitation Salting in / Salting out Salting IN low concentrations of salt usually increases the solubility of charged macromolecules. Salting OUT high concentrations of added salt lowers the solubility of and they come out of solution. Effect of K2SO4 on the solubility of Hb-CO 离子强度 溶解度 1) electrophoresis电泳 charged proteins move toward the opposite electrode. Many kinds of different electrophoresis methods 3 . Based on charge difference 聚丙烯酰胺凝胶电泳 等电聚焦/isoelectric focusing, IEF 2-dimensional el