T细胞淋巴瘤中组织蛋白酶D和E.docxVIP

鼻NK/T细胞淋巴瘤中组织蛋白酶D和E【关键词】 鼻肿瘤；淋巴瘤,T细胞；杀伤细胞,天然；免疫组织化学；原位杂交；组织蛋白酶D；E　　Expressions and significances of cathepsin D and Eselectin in nasal NK/T cell lymphoma　　【Abstract】 AIM: To explore the expressions and significances of cathepsin D (CD) and Eselectin in the newly recognized nasal NK/T cell lymphoma. METHODS: Immunohistochemical SP method was used in 41 cases of nasal nonHodgkin s lymphoma to examine the expressions of CD45RO, CD3ε, CD20, CD79α, CD56 and TIA1; In situ hybridization for EBER1/2 (EBERISH) was used to examine EpsteinBarr virus (EBV) infection. As a result, 21 cases of nasal NK/T cell lymphoma were diagnosed according to the newly published WHO standard. The expressions of CD and Eselectin were detected immunohistochemically in the 21 cases of nasal NK/T cell lymphoma and 10 cases of lymphoid hyperplasia. RESULTS: CD was expressed intensively in plasma of scattered stromal histiocytes and the positive signal of CD in nasal NK/T cell lymphoma was much stronger than that in lymphoid hyperplasia （t=39.12, P＝0.000）. The positive signal of Eselectin was expressed in vascular endothelium and the mean positive rates of the 2 groups had no significant difference statistically(t=0.688, P＝0.497). CONCLUSION: The higher expression of CD in the stroma of nasal NK/T cell lymphoma may relate to the high aggressiveness of nasal NK/T cell lymphoma, while Eselectin may have no intimate correlation with it.　　【Keywords】 nasal neoplasms; lymphoma, T cell; killer cells, natural; immunohistochemistry; in situ hybridization; cathepsin D; Eselectin　　【摘要】 目的： 探讨组织蛋白酶D（CD）和E选择素(Eselectin)在鼻NK/T细胞淋巴瘤中的表达及意义. 方法： 采用免疫组化SP法检测41例鼻部非霍奇金恶性淋巴瘤CD45RO, CD3ε, CD20, CD79α, CD56和TIA1的表达；EBER原位杂交法检测EB病毒感染情况，根据最新WHO分类及诊断标准诊断鼻NK/T细胞淋巴瘤；对完全符合诊断标准的21例鼻NK/T细胞淋巴瘤和10例鼻咽增生淋巴组织活检标本，用免疫组化方法检测CD和E选择素的表达. 结果： 鼻NK/T细胞淋巴瘤间质中组织细胞CD阳性信号的表达明显高于增生淋巴组织组(t=39.12, P＝0.000）；E选择素表达于血管内皮细胞，鼻NK/T细胞淋巴瘤与鼻咽增生淋巴组织中E选择素平均血管阳性率差异无统计学意义（t=0.688, P＝0.497）. 结论： 鼻NK/T细胞淋巴瘤间质中CD的表达明显增高，与该肿瘤高度侵袭性相关；E选择素与鼻NK/T细胞淋巴瘤恶性生物学行为无直接相关性