核因子.docxVIP

核因子 作者：余杨,何德沛,申林,吴洪坤 【摘要】 目的 探讨体外循环(ECC)时核因子-κB（NF-κB）与全身炎性细胞因子的变化及关系。方法 选择当地健康杂种犬，常规建立体外循环动物模型。实验组于术前将吡咯二硫氨基甲酸酯（PDTC）20 mg/kg溶解在生理盐水中静脉推注，而对照组仅给予同等剂量的生理盐水。全部实验动物在围体外循环期各时间点分别取相应标本进行NF-κB及血清肿瘤坏死因子-α(TNF-α)、白介素(IL)-6、IL-8的检测。结果 ECC介入后，实验组在各时间点NF-κB基因表达水平及血清炎性细胞因子浓度的增高都明显低于对照组。结论 ECC会引起NF-κB量及血清炎性细胞因子浓度的增加，通过抑制NF-κB能显著减轻ECC术后炎性细胞因子增加的程度。【关键词】 体外循环；核因子-κB；细胞因子；抗氧化剂　　The Study of Relationship between Nuclear Factor-κB and Inflammatory Response to Extracorporeal Circulation　　Abstract: OBJECTIVE To investigate the changes and relationship of nuclear factor-κB(NF-κB) and inflammatory cytokine in extracorporeal circulation(ECC). METHODS Routine ECC was established in adult mongrels. Pyrrolidine dithiocarbamate(PDTC) was administered via vein before operation in experimental group and saline was given in control group. In all animals, the corresponding samples were obtained perioperatively. NF-κB mRNA expression, serum tumor necrosis factor(TNF)-α, interleukin(IL)-6、IL-8 were measured respectively. RESULTS Compared with control group, the increased productions of TNF-α、IL-6、IL-8 and activation of NF-κB were significantly suppressed in experimental group after ECC. CONCLUSION ECC can induce the increasement of NF-κB mRNA expression and serous cytokine level. Through inhibiting NF-κB activation, it can significantly attenuate inflammatory cytokine after ECC.　　Key words： Extracorporeal circulation;Inflammatory Response;Nuclear Factor-κB;Cytokine;Antioxidant　　体外循环（extracorporeal circulation，ECC）时炎症的触发及其级联反应内部相互作用十分复杂，存在多条不同的炎症通路及复杂的分子网络，通过众多细胞信号转导途径激发炎性反应。目前，国内外众多临床及实验研究均指出核因子-κB(NF-κB)是此过程的核心［1］。故我们在活体狗ECC动物模型上使用其特异性抑制剂-吡咯二硫氨基甲酸酯（PDTC），观察其在围ECC期间对NF-κB激活和炎性细胞因子的影响。　　1 资料与方法　　1.1 实验动物及ECC方法 当地健康杂种犬随机分为实验组和对照组（每组6只），体重12～15 kg。按1 ml/kg体重腹腔注射2.5%戊巴比妥钠麻醉［2］，术中麻醉减浅时可按半量静脉推注以维持。静脉给维库溴铵（0.2 mg/kg）诱导插管［3］，接麻醉呼吸机。监测心率、有创动脉压。实验组于术前10 min将PDTC（20 mg/kg）溶解在10 ml生理盐水中静脉推注，而对照组仅给予同等剂量的生理盐水。经左侧肋间进胸，常规建立ECC。两组动物模型平均ECC时间约60 min。　　1.2 监测指标方法 全部实验动