结构油脂资料台湾.pdfVIP

中文摘要 人類母乳脂肪中含有 20-25%的棕櫚酸 (C16:0) 以及 30-35%的油酸 (C18:1) ，其中大約 70%的棕櫚酸鍵結在三酸甘油酯的第二個 位置上。嬰兒食品中主要的結構脂質為 1,3-dioleoyl-2-palmitoyl- glycerol(OPO) 。本研究以三個步驟合成結構脂質OPO ，(一)將棕櫚 酸與甘油以 Lipozyme IM催化進行酯化反應，可得到酯化程度 98.8% ，產物甘油酯中三酸 甘油酯含量為 98.62% 。經矽膠管柱純化後，可得100%的三酸甘油酯，其脂肪酸組成 99% 為棕櫚酸。(二)從橄欖油為原料經兩階段低溫溶劑結晶法，可將橄欖油脂肪酸中油酸由原來 之 76.76%提昇至 96.64% ，總回收率達 72.84% 。(三)將上述三棕櫚酸甘油酯與油酸以 Lipozyme IM 催化進行酸解反應以合成結構脂質 OPO ，在產物甘油酯中油酸之含量可達 66.07 mol% ，三酸甘油酯佔97.12% ，結構脂質OPO 在酸解產物三酸甘油酯中佔 74.26 mol% 。 關鍵詞：母乳脂肪、棕櫚油、棕櫚酸、油酸、濃縮、結構脂質 I Abstract Human milk fat contains 20-25% palmitic acid (C16:0), 30-35% oleic acid (C18:1) and about 70% of the palmitic acid is esterified to the sn-2 position of triacylglycerols. An important structured lipid in infant formula is 1,3-dioleoyl-2-palmitoyl-glycerol (OPO). The object of this study is to synthesize structured lipid OPO. Firstly, esterification of palmitic acid and glycerol catalyzed by Lipozyme IM, gave 98.8% degree of esterification with a triacylglycerol content of 98.62% in the product. Separation of the reaction mixture by silica gel column chromatography gave 100% triacylglycerol which contained 99% palmitic acid. Secondly, the purification of oleic acid from olive oil by two-stage low temperature solvent crystallization, oleic acid content in olive oil-fatty acid can be raised from 76.76