旧版pfge脉冲场电泳技术培训-4chef故障排除20140224.pptVIP

Old culture 48 hrs Fresh culture 18 hrs PFGE gel from fresh culture shows no smearing and all bands are distinct. Lysed cells are present in 48 hour old cultures (same organism), characterized by smears on the PFGE gel (blue arrow). Potential problems: Undue stress to bacterial cells prior to casting the plug can cause cell lysis, leading to DNA degradation and is characterized by “smearing” on the gel. Recommendations: Grow cultures on non-selective media Use fresh cultures grown for 14 – 18 hours at appropriate temperature and conditions Do not let cultures sit at room temp for more