CRISPRCas9基因组编辑技术及其在动物基因组定点修饰中方案.PDFVIP

Hereditas (Beijing) 2015 10 , 37(10): CRISPR/Cas9 1 1 2 1 1 1. 611130 2. 100101 CRISPR/Cas CRISPR RNAs (crRNAs)Cas CRISPR/Cas (Zinc-finger nucleases, ZFNs) TALE (Transcription activator like effector nucleases, TALENs)CRISPR/Cas9 CRISPR/Cas CRISPR/Cas9 CRISPR/Cas9 CRISPR/Cas9 genome editing technique and its application in site-directed genome modification of animals 1 1 2 1 1 Jinwei Zhou , Qipin Xu , Jing Yao , Shumin Yu , Suizhong Cao 1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Abstract: CRISPR/Cas system, which uses CRISPR RNAs (crRNAs) to guide Cas nuclease to silence invading nucleic acids, is self-defense system against exogenous virus or plasmid in bacteria and archaea. Through molecular modification, the type CRISPR/Cas system has become a highly efficient site-directed genome editing technique, which is simpler than zinc-finger nucleases (ZFNs) and transcription activator like effector nucleases (TALENs) and easier to be designed and applied. In this review, we summarize the evolutionary history of CRISPR/Cas9 system, the working principle and modification process of type CRISPR/Cas and its application in animal genome modifica- tion. We also analyze the existing problems and improvement program of the CRISPR/Cas9 sy stem as well as its ap- plication prospect combined with successful cases, which may provide innovative perspectives on improving animal traits and establis