海绵放线菌分离及抑菌活性筛选探究.docVIP

海绵放线菌分离及抑菌活性筛选探究 　　摘要 本文用5种分离培养基从大连海域潮间带采集的2种海绵中分离培养放线菌，以副溶血弧菌、哈维弧菌和鳗弧菌3种海水养殖病原菌为指示菌对放线菌进行抑菌活性的筛选，并基于16S rDNA序列对筛选出的抑菌活性较强的海绵放线菌进行多样性分析。结果表明：从海绵中共分离出57株海绵放线菌，以寡营养的M2培养基分离效果最好。抑菌活性筛选有55株对3株指示菌中的至少1株具有抑菌活性；有11株抑菌活性较强，对3株指示菌均具有抑菌活性，初步鉴定均属于链霉菌属。由此表明，海绵放线菌多能够产生对海水养殖病原菌较强的抑菌活性，是天然活性物质的重要来源，在海水养殖应用方面具有较大的开发价值 关键词 海绵放线菌；抑制弧菌；抑菌活性；多样性分析 中图分类号 Q939.13 文献标识码 A 文章编号 1007-5739（2017）07-0229-03 Isolation of Sponge-associated Actinobacteria and Its Antibacterial Activity Screening DU Ying-xia 1 REN Hao-jie 1 ZHANG Ju-lin 2 FU Wan-tao 2，3 * （1 College of Fisheries and Life Science，Dalian Ocean University，Dalian Liaoning 116023； 2 College of Marine Science-Technology and Environment，Dalian Ocean University； 3 Key Laboratory of Nearshore Marine Environmental Science and Technology of Liaoning Province′s University） Abstract This text isolated actinomycetes from 2 kinds of sponge taken from Dalian ocean intertidal zone with 5 kinds of separation medium.The antibacterial activity of sponge-associated actinobacteria was screened with Vibrio parahaemolyticus，Vibrio harveyi and Vibrio anguillarum as indicator bacteria.The diversity analysis of sponge-associated actinobacteria with strong antibacterial activity was conducted based on 16S rDNA sequence.The study showed that 57 strains in total were isolated from the sponges，and the separation effect of oligo-culture M2 was the best.The result of antibacterial activity screening found that 55 strains had antibacterial effect on at least one of the 3 indicator bacteria.There were 11 strains preliminarily identified as Streptomyces sp. having stronger antibacterial effect on all the 3 indicator bacteria.The results showed that most of the sponge-associated actinobacteria which was an important source of bioactive natural products had quite strong inhibition on mariculture pathogen.It has a great development value in the field of mariculture. Key words sponge-associated actinobacteria；inhibition of vibrio；antibacterial activi