遗传学报ActaGenetica.PDFVIP

遗 传 学 报 Acta Genetica Sinica , December 2006, 33 (12)：1081–1086 ISSN 0379-4172 Analysis of the Genetic Diversity and the Phylogenetic Evolu- tion of Chinese Sheep Based on Cyt b Gene Sequences WANG Xin1,2, MA Yue-Hui2,①, CHEN Hong 1,3,① 1. College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 3. Institute of Biotechnology, Xuzhou Normal University, Xuzhou 221116, China Abstract: The complete sequences of Cyt b gene from 20 individuals belonging to eight Chinese indigenous sheep breeds and one foreign breed were studied. The results showed that the hapolotype diversity of Chinese sheep breeds was 97.1%. The mean nucleotide composition of all the sequences was 27.1% T, 28.5% C, 31.4% A, and 13.0% G. The nucleotide diversity was 0.602%. A total of 43 mutation sites were detected, including 40 transitions and 3 transversions. Fu’s test of selective neutrality showed that the sheep populations had no population demographic expansion (0.10 P 0.05). The different clustering methods, namely neighbor-joining, minimum evolution, and unweighted pair group method with arithmetic means, all showed a similar result, which indicated that Chinese local sheep had three maternal resources. Key words: sheep; Cyt b; phylogenetic evolution Mitochondrial DNA (mtDNA) is the genetic opment of animal husbandry and for the preservation material that exists outside the nucleus in eukaryotic of biological diversities[4]. For example, Ganjia and cells. It has a simple molecular structure. It does not Oula sheep in Gansu Province belonging to local undergo recombinati