阿尔茨海默病转线粒体DNA细胞模型胞质钙稳态的改变-首都医科大学.PDFVIP

2011 2 F eb. 20 11 32 1 Journal of C apitalM ed ical U niversity V o l. 32 N o. 1 [ do:i 10. 3969/ j. issn. 2011. 01. 014] 阿尔茨海默病转线粒体 DNA 细胞模型胞质钙稳态的改变 1, 2 1, 3 1, 2 1, 2 1, 2* 张 兰 刘 芳 万岁桂 贾建平 李 林 1. ; 2. ; 3. ) A lzhemi er s d isease, AD ) DNA , DNA m itochondr ia l DNA, m tDNA ) A D AD m tDNA , m tDNA C cytochrom e C ox idase, COX ) ; F luo3, AD m tDNA COX P 0. 05) ; , CCCP A D COX , , ; ; DNA ; C; R 395. 2; R 741; R 338 A lteration of Cytosoli Cal ium in m tDNAtransferred Cells of A lzheim er s D isease 1, 2 1, 3 1, 2 1, 2 1, 2* ZHANG L an , L IU F ang , WAN Su igu i , JIA Jianp ing , L I L in 1. D ep artm ent of Pharm acology, X uanwu H osp ita ,l Cap italM ed ical University; 2. K ey La oratory f orN eurodegenerative D iseases, M inis try of Education; 3. D epartm ent of Pharmacy, N o. 3 H osp italAff iliated to B eij ing University ) AB STRACT Obje tive T o investiga te the leve l o f cytoso lic free ca lc ium in m itochondr ia DNA transferred ce lls of A lzhe mi er s d isease and the influence o fm itochondria lDNA m tDNA ) def ic iency on ca lcium regu lation capac ity o f cells. M ethods P late lets from AD patients and aged contro l persons w e re fu sed w ith m tDNA depleted ce lls to deve lop m tDNAtransferred cellm ode.l Cytochrom e C ox idase COX ) ac tiv ity w as de term ined by m ic roplate assay; cy toso lic ca lcium w as labeled w ith F luo3 and fluo rescence density w as detected w ith laser scan con foc