第四章 病毒感染组织特异性研究.pdf

Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus † ‡ ‡ Katsuhiko Ito* , Kurako Kidokoro*, Hideki Sezutsu , Junko Nohata*, Kimiko Yamamoto*, Isao Kobayashi , Keiro Uchino‡ ‡ † † , Andrew Kalyebi*, Ryokitsu Eguchi*, Wajiro Hara*, Toshiki Tamura , Susumu Katsuma , Toru Shimada , Kazuei Mita*, and Keiko Kadono-Okuda*§ *Division of Insect Sciences, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan; †Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan; and ‡Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan Edited by Barry J. Beaty, Colorado State University, Fort Collins, CO, and approved March 14, 2008 (received for review December 17, 2007) Bombyx mori densovirus type 2 (BmDNV-2), a parvo-like virus, 034C07 and the SP6 end of BAC clone 001K10 [supporting replicates only in midgut columnar cells and causes fatal disease. information (SI) Datasets S1 and S2]. The region was 400 kb The resistance expressed in some silkworm strains against the virus in length and covered by four BAC clones, 022C09, 067M09, is determined by a single gene, nsd-2, which is characterized as 021B10, and 059I11 (Fig. 1C). nonsusceptibility irrespective of the viral dose. However, the In the comparison between resistant (J150) and susceptible responsible gene has been