应用重组钙离子敏感蛋白yc21研究裂殖酵母胞内钙离子浓度的分布.pdfVIP

蛊塞哑蒸占坐蠛±生焦堑窑 重旦重塑登融E缝盛重鱼)里2：{l醚窿型壁嫠堡融杰!!盥王篷廑茧盘盔 离子浓度坟商。这提示钙离子可能与裂殖酵母细臌分裂有关，但是需要进一步的 实验证弱。 关键词：粟酒裂殖酵母，钙离子敏感蛰I刍YC2．1，细胞内游离钙离子，fluo-3 壹直韪器盘堂璧圭堂壁鸷塞 瘗照蘸塑篓矗王墼整爆鑫地』翌}基型壁鳇堡缒盎丛照王照塞煎垒生 Abstract etal YC2，l isaliartiflcal Tsien in1997。ItS gene geneby expression product …一YC2．1 callcombine讯mcalcium and protein its日uoresceoce．It reverselychmlge canindicate calcium anddistributioneonfocal cytoplasmdensity using microscope． Aswecarlfuse with YC2．1 ofYC2。l gene signal—peptidesequence。theexpression Carlbe methodYC2l protein orientated using todetectcalcium exactly．The protein is inrecent thereisno abouttheresearch appliedrapidly years．But report using YC2．1 inSchizosaccharomycesporebe． Inthis observedthecalciumdistributionGf in paper，vce cytoplasm Spombe。 two Wetake totransformtheYC2．1 intothe make steps gene S．pombe．Firststep，we orientation fiat one endandone end we stickingligation．And insertingby get has code．YC2．1and pESP-YC2．1’plasmid．T鞋sphsmidpromoter,start gene terminatorbut use soft,,vare no code．Second OLIGO to