nc hf cr r400 r50 apoptosis by tunel (×200) sirt1 mrna .ppt

* Baur JA, et al. Nature, 2006, 444(7117):337-42 Lagouge M, et al.Cell, 2006, 127(6):1109-22 Reverse diabetes induced by a high-fat diet * * Caloric Restriction and Resveratrol Reverse a High-fat Diet Induced Diabetes and Improve Islet β cell Dysfunction in Mice Jiaoyue Zhang Ph.D Endocrinology Department,Union hospital, Huazhong University of Science Technology, Wuhan 430022, China Caloric Restriction and Resveratrol Calorie restriction (CR), based on low calorie intake, is basic for therapy of diabetes SIRT1 is the NAD+-dependent deacetylase, and controls the activities of genes that regulate circadian rhythm, apoptosis. Resveratrol (Res), a type of natural phenol, is the potent natural SIRT1 activator. It has been found to lower plasma glucose in streptozotocin-induced diabetic rats. SIRT1 is involved in the activities of β cell SIRT1 was preferentially expressed in pancreatic beta cells while not in exocrine cells In beta cell-specific Sirt1-overexpressing (BESTO) transgenic mice，GSIS ↑； SIRT1 reduced by siRNA in beta cel lines (INS, MIN6) ，GSIS↓ Our hypothesis： SIRT1 could possibly play an important role in the effect of CR and Res on beta cell Bordone L, et al. PLoS Biol, 2006,4:e31 Moynihan KA, et al. Cell Metab, 2005,2:105-17 Aim of the research Explore the anti-hyperglycaemic role and mechanisms of CR and Res on beta cell protection male C57BL/6J mice control model standard chow ×24w Normal control High fat diet Large dosage of Res Low dosage of Res Caloric restriction NC HF CR R400 R50 15 65 High fat diet×8w standard chow +60%CR a high fat diet ×16w a high fat diet +Res400mg/kg/d a high fat diet +Res50mg/kg/d IPGTT Caloric intake、BW、lipid IPGTT、insulin、ITT 15只 Obesity index＝（total fat weight）/body weight×100 Compared to NC, *P0.05，#P0.01；compared to HF, △P0.05，▲P0.01 Caloric intake Body weight Blood FFA Obesity index # #▲ #▲ *▲ #▲ #▲ # # # * ▲ #▲ # ▲ 8w 24w IPGTT curve FBG AUC # ▲ *△ ▲ Fasting blood insulin HOMA-IR Insulin tolerance (ITT) K