基于准静态平面流场的新型皮肤组织工程 - 第三军医大学学报.docVIP

基于准静态平面流场的新型皮肤组织工程 灌注式生物反应设计与初步模拟 王洋1，蔡伟华2，伍津津1，唐辉1，杨涛1，唐书谦1 1 第三军医大学大坪医院野战外科研究所皮肤科，重庆市创伤愈合与组织工程研究所，重庆400042； 2 哈尔滨工业大学能源科学和工程学院，哈尔滨150001 摘要：目的 首次提出并设计了准静态平面流场的灌注式新型生物反应器。方法 首次采用计算流体动力学（CFD）商业软件FLUENT模拟新型生物反应器中的流场及培养支架壁面上的切应力。结果 模拟结果表明筛网状支撑物及组织工程皮肤构建物表面处的最大切应力小于3dyn/cm2，且培养室中的流场是均匀的。该反应器克服了其他生物反应器流场不均匀性、体积过大等，适合皮肤培养气液界面特殊要求。结论 新型生物反应器满足准静态平面流场的要求，有可能在未来的皮肤组织工程研究和生产中广泛用。 关键词：新型灌注式生物反应器；皮肤组织工程；计算流体力学；准静态平面流场 The design and simulation of a new perfusion bioreactor for tissue-engineered skin based on the quasi-static plane flow Yang Wang1, Wei Hua Cai2, Jin-Jin Wu1,( Hui Tang1, Tao Yang1, Shu-qian Tang1 1 Department of Dermatology, Research Institute of Wound Healing and Tissue Engineering of Chongqing, Daping Hospital, Third Military Medical University, Chongqing, 40042, China. 2 School of Energy Science and Engineering, Harbin Institute of Technology, Harbin, 150001, China Abstract: Objective In this paper, it is firstly proposed the idea of the quasi-static plane flow for the perfusion culture and designed the new bioreactor for culturing engineered-skin structures (ESS). Methods Computational fluid dynamics analysis of the new bioreactor was firstly performed to characterize the flow field and wall shear stress (WSS). It had some advantages including stack-up, low stress and homogeneous flat flow field. Result The results showed that the maximum WSS in the growth chamber is less than 3dyn/cm2 and the flow field in the bioreactor is uniform. The new bioreactor remedies some disadvantages appeared in other bioreactors, such as the heterogeneous characteristics of flow, the excess volume etc. Conclusion It suggests that the new bioreactor satisfies the demand of quasi-static plane flow and it may be suitable for culturing and producing the tissue-engineered skin in the future. Keywords: New perfusion bioreactor, tissue engineered skin, Computational fluid dynamics, quasi-static plane flow Corresponding author: Jin-Jin W