不同提取溶剂对独一味药材中木犀草素含量的影响_徐梦雪.pdfVIP

不同提取溶剂对独一味药材中木犀草素含量的影响 1＊ 2# 徐梦雪 ，王贤英 （1.重庆医科大学附属儿童医院，重庆 400014；2.重庆市中药研究院，重庆 400065 ） 中图分类号 R927.2 文献标志码 A 文章编号 1001-0408（2015）03-0391-03 DOI 10.6039/j.issn. 1001-0408.2015.03.33 摘 要 目的：研究不同提取溶剂对独一味药材中木犀草素含量的影响。方法：采用高效液相色谱法测定木犀草素的含量，分别 考察水、50％乙醇、70％乙醇回流提取方法制得的供试品溶液中木犀草素的含量。色谱柱为Diamonsil C18，流动相为甲醇-0.4％磷 酸溶液（50∶50，V/ V），检测波长为350nm ，柱温为25℃，进样量为10μl。结果：木犀草素进样量在0.0408～0.2040μg 范围内与其 峰面积积分值呈良好线性关系（r ＝0.9998）；精密度、稳定性、重复性试验的RSD ≤1.12％；平均加样回收率为98.68％，RSD 为 0.84％（n ＝6）；水、50％乙醇、70％乙醇回流提取制备的供试品溶液中木犀草素的含量分别为3.93、4.75、5.93mg/g 。结论：不同提 取溶剂制备的供试品溶液中独一味药材中木犀草素的含量不同，从高到低依次为70％乙醇＞50％乙醇＞水。本方法操作简便、 结果可靠，可用于独一味药材中木犀草素的含量测定。 关键词 独一味；木犀草素；高效液相色谱法；提取溶剂 Effects of Different Extraction Solvents on the Content of Luteolin in Lamiophlomis rotate 1 2 XU Meng-xue ，WANG Xian-ying （1.Children ’s Hospital of Chongqing Medical University ，Chongqing 400014， China ；2.Chongqing Academy of Traditional Chinese Medicine ，Chongqing 400065，China ） ABSTRACT OBJECTIVE ：To study the effects of different extraction solvents on the content of luteolin in Lamiophlomis rotate . METHODS ：The content of luteolin in L. rotate was determined by HPLC. The contents of luteolin in test samples prepared by wa- ter exlraction ，50％ alcohol exlraction and 70％ alcohol exlraction were all determined. The determination was performed on Di- amonsil C18 column with mobile phase consisted of methanol-0.4％phosphoric acid （50∶50，V/ V）. The detection wavelength was set at 350 nm and detection temperature was 25 ℃，the sample size was 10 μl. RESULTS ：The linear range of luteolin were 0.040 8-0.204 0 μg （r ＝0.999 8）with an average recovery of 98.68％（RSD ＝0.84％，n ＝6）. RSDs of precision ，reproducibility and stabil- iy tests were lower than 1.12％. The contents of luteolin in test samples prepared by water exlraction ，50％alcohol exlraction and 70％alcohol exlraction