ELISA实验基础知识培训.pptxVIP

ELISA实验基础知识培训;1. 酶联免疫吸附实验（ELISA）基本原理及概念;第一部分 酶联免疫吸附实验（ELISA）基本原理及概念;免疫技术;5;6;7;8;9;10;11;12;13;14;15;16;17;18;;20;采用辣根过氧化物酶的原因;22;23;24;;ELISA方法的适用范围;;28;29;甲肝疫苗抗原检测的方法——双抗体夹心法;第二部分 酶联免疫吸附实验（ELISA） 定量检测应用;目录;Guidance for Industry Bioanalytical Method Validation;34;Precision should be measured using a minimum of five determinations per concentration. A minimum of three concentrations in the range of expected concentrations is recommended. The precision determined at each concentration level should not exceed 15% of the coefficient of variation (CV) except for the LLOQ, where it should not