罗丹明B强吸附分子印迹材料的制备及吸附性能评价-食品安全质量检测.docVIP

罗丹明B强吸附分子印迹材料的制备及吸附性能评价 刘静静, 肖申鸿, 张 伟, 严 恒, 谢卫红* (1. 湖北工业大学生物工程与食品学院, 武汉 430068; 2. 湖北省食品质量安全监督检验研究院武汉430073) 摘 要: 目的 制备一种对罗丹明B具有特异性和强吸附性能的分子印迹材料。方法采用沉淀聚合法合成罗丹明B分子印迹聚合物(molecularly imprinted polymer, MIP), 对分子印迹材料的动力学吸附、等温饱和吸附、选择性吸附及其对果汁样品中罗丹明B的吸附能力进行评价。结果以丙烯酰胺为功能单体制备的印迹聚合物的印迹效率(a=QMIP/QNIP)可达3。动力学吸附实验表明, MIP对罗丹明B的吸附可在15 min内完成。等温吸附性能评价得出饱和吸附量约为170 μg/mg。罗丹明B分子印迹聚合物对几种红色色素的吸附量的顺序为: 罗丹明B诱惑红赤藓红苋菜红胭脂红。罗丹明B分子印迹聚合物对于添加到果汁中的罗丹明B可以很好地吸附而对其他成分的吸附较小。结论罗丹明B分子印迹聚合物对罗丹明B具有明显的特异性吸附性能, 而且具有较强的吸附能力, 是一种具有潜力的用于罗丹明B分析的固相萃取材料。 关键词罗丹明B分子印迹聚合物分子印迹技术固相萃取 Synthesis of molecularly imprinted polymer for strong adsorption of rhodamine B and the evaluation of its adsorption abilityLIU Jing-Jing, XIAO Shen-Hong, ZHANG Wei, YAN Heng, XIE Wei-Hong* (1. College of Bioengineering and Food Science, Hubei University of Technology, Wuhan 430068, China;2. Hubei Provincial Institute for food Supervision and Test, Wuhan 430073, China) ABSTRACT: Objective To synthesize a molecularly imprinted polymer (MIP) with strong and specific adsorption ability for rhodamine B (RhB). Methods Rhodamine B imprinted polymer was synthesized using a precipitate polymerization method. The adsorption ability of the MIP was evaluated by the kinetic adsorption experiment, isothermal adsorption experiment, adsorption selectivity experiment and the adsorption ability of MIP to rhodamine B in juice sample. Results The imprinted factor of the molecularly imprinted polymer was 3 with acrylamide as functional monomer. It indicated from kinetic adsorption experiment that the MIP could reach a saturated adsorption within 15 min. The isothermal adsorption experiment showed the maximum adsorption was 170 μg/mg. The adsorption capacity of the MIP for some red pigments followed an order of rhodamine B temptation red erythrosine amaranth carmine. RhB-MIPs could adsorb for rhodamine B added to fruit juice well while the adsorption to other components was small. Conclusion The rhodamine B imprinted polymer shows a strong an