斜纹夜蛾核多角体病毒郴州株的生物学与应用研究 - 中国烟草科学.PDF

2012-02 ，33 （1） Chinese Tobacco Science 81 1 2 2 1 2* 1 2 2 1 1. 423000 2. 423000 Spodoptera litura nucleopolyhedrovirusSpliNPV 65.0% 48 d 86.6% 88.4% 1.03×1011 OBs/mL S435.72 1007-5119201201-0081-04 DOI 10.3969/j.issn.1007-5119.2012.01.018 Biology and Application of Spodoptera litura nucleopolyhedrovirus Chenzhou Strain 1 2 2 1 2* 1 2 LI Xiaoyi , LI Hongguang , CAO Jian , LUO Jingren , ZENG Weiai , ZHOU Linghong , KUANG Zhuanfu , 2 1 CHENG junping , LI Yunxia (1. Chenzhou Institute of Agricultural Sciences, Chenzhou, Hunan 423000, China; 2. Chenzhou Base, South-central Agricultural Experiment Station, CNTC, Chenzhou, Hunan 423000, China) Abstract: The laboratory bioassay and field control efficacy of Spodoptera litura nucleopolyhedrovirus (SpliNPV) Chenzhou strain were preliminarily examined. The efficient artificial propagation method was used to feed the host larvae with virus suspension, the average mortality of the insects was 65.0%. The death peak of the pests was in 4-8 days after infecting the virus. The high temperature and humidity, and poor light could help this virus. Filed control efficacy of Chenzhou strain was 86.6% in laboratory, which was better than another commercial strain. The corrected control efficacy of this strain was 88.4% in the fields, which was higher than that of avermectin pesticide significantly. It was detected that the occlusion body (OB) concentration of the initial virus’s stock solution was 1.03 ×1011 OBs/ ml, and it was a strong SpliNPV stain, as it showed an excellent efficacy to control the pest Spodoptera li