创伤性脑损伤大鼠海马microRNA表达研究.docVIP

  Expression profiling of microRNAs in hippocampus of rats following traumatic brain injury# SUN Tingyi, LIU Zilong, LIU Liang, CHEN Xiaorui, ZHAO Lili, QU Guoqiang** 5 10 15 20 25 30 35 40 45 (Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, WuHan 430030) Abstract: Objectives To descript the changes in expression of microRNAs in the hippocampus of rats at 1 day, 3 days and 5 days following traumatic brain injury (TBI) by microarray technique, and investigate the possible cellular activities that are regulated by microRNAs which differentially expressed in the hippocampus, that may contribute to TBI-induced cognitive impairment. Methods Adult SD rats received a single controlled cortical impact (CCI) injury using an electromagnetic piston at a velocity of 4 m/sec, duration of 150 ms and depth of 3 mm. The ipsilateral hippocampus was harvested for the subsequent microarray assays at three time points post-injury: 1 day, 3 days and 5 days, respectively. We characterize the microRNA expression profile in rat hippocampus compared with sham group using the Significance Analysis of Microarrays analysis. Results We got a dendrogram of a Hierarchical Clustering analysis of the result. Totally 205 microRNAs were identified which were up-/down-regulated more than 1.5 times. At 1 d post-injury, 41 microRNAs were up-regulated more than 1.5 times, and 31 microRNAs were down-regulated more than 1.5 times; at 3 d post-injury, 81 microRNAs were up-regulated more than 1.5 times, and 11 microRNA s were down-regulated more than 1.5 times; at 5 d post-injury, 52 microRNAs were up-regulated more than 1.5 times, and 41 microRNAs were down-regulated more than 1.5 times. Furthermore, we revealed 17 microRNAs which were changed at all three time points post-injury, the notable two microRNAs were miR-142-3p and miR-221, which could involve in many pathophysiological processes. Conclusions Our microarray-based bioinformatics analysis